The impact of breed and tissue compartment on the response of pig macrophages to lipopolysaccharide

BMC Genomics

Research Article

Dario Beraldi¹  Christopher K Tuggle²  David P Sester³  Lynsey Fairbairn⁴  Alison Downing⁵  Tom C Freeman⁵  David A Hume⁵  Alan L Archibald⁵  Ronan Kapetanovic⁵ 

[1] Cancer Research UK Cambridge Research Institute, Li Ka Shing Centre, Robinson Way, CB2 0RE, Cambridge, UK;Department of Animal Science, Iowa State University, 50011, Ames, IA, USA;Innate Immunity Laboratory, School of Chemistry and Molecular Biosciences, University of Queensland, QLD 4072, Brisbane, Queensland, Australia;Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München, 81679, Munich, Germany;The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, EH25 9RG, Midlothian, Edinburgh, United Kingdom;

关键词: Pig;    Macrophages;    Microarray;    Breed;    Lipopolysaccharide;   

DOI  :  10.1186/1471-2164-14-581

 received in 2013-04-04, accepted in 2013-08-06,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundThe draft genome of the domestic pig (Sus scrofa) has recently been published permitting refined analysis of the transcriptome. Pig breeds have been reported to differ in their resistance to infectious disease. In this study we examine whether there are corresponding differences in gene expression in innate immune cellsResultsWe demonstrate that macrophages can be harvested from three different compartments of the pig (lungs, blood and bone-marrow), cryopreserved and subsequently recovered and differentiated in CSF-1. We have performed surface marker analysis and gene expression profiling on macrophages from these compartments, comparing twenty-five animals from five different breeds and their response to lipopolysaccharide. The results provide a clear distinction between alveolar macrophages (AM) and monocyte-derived (MDM) and bone-marrow-derived macrophages (BMDM). In particular, the lung macrophages express the growth factor, FLT1 and its ligand, VEGFA at high levels, suggesting a distinct pathway of growth regulation. Relatively few genes showed breed-specific differential expression, notably CXCR2 and CD302 in alveolar macrophages. In contrast, there was substantial inter-individual variation between pigs within breeds, mostly affecting genes annotated as being involved in immune responses.ConclusionsPig macrophages more closely resemble human, than mouse, in their set of macrophage-expressed and LPS-inducible genes. Future research will address whether inter-individual variation in macrophage gene expression is heritable, and might form the basis for selective breeding for disease resistance.

【 授权许可】

Unknown   
© Kapetanovic et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.