Luminal and basal-like breast cancer cells show increased migration induced by hypoxia, mediated by an autocrine mechanism

BMC Cancer

Research Article

Desmond G Powe¹  Kurt S Zänker²  Melanie J Voss²  Frank Entschladen²  Mischa F Möller²  Bernd Niggemann² 

[1] Department of Cellular Pathology, Queen's Medical Centre, Nottingham University Hospitals NHS Trust, NG7 2UH, Nottingham, UK;Institute of Immunology, ZBAF, Witten/Herdecke University, Stockumer Str. 10, 58448, Witten, Germany;

关键词: Conditioned Medium;    Migratory Activity;    Neutrophil Granulocyte;    Oxygen Deprivation;    Blebbistatin;   

DOI  :  10.1186/1471-2407-11-158

 received in 2010-08-17, accepted in 2011-05-02,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundSome breast cancer patients receiving anti-angiogenic treatment show increased metastases, possibly as a result of induced hypoxia. The effect of hypoxia on tumor cell migration was assessed in selected luminal, post-EMT and basal-like breast carcinoma cell lines.MethodsMigration was assessed in luminal (MCF-7), post-EMT (MDA-MB-231, MDA-MB-435S), and basal-like (MDA-MB-468) human breast carcinoma cell lines under normal and oxygen-deprived conditions, using a collagen-based assay. Cell proliferation was determined, secreted cytokine and chemokine levels were measured using flow-cytometry and a bead-based immunoassay, and the hypoxic genes HIF-1α and CA IX were assessed using PCR. The functional effect of tumor-cell conditioned medium on the migration of neutrophil granulocytes (NG) was tested.ResultsHypoxia caused increased migratory activity but not proliferation in all tumor cell lines, involving the release and autocrine action of soluble mediators. Conditioned medium (CM) from hypoxic cells induced migration in normoxic cells. Hypoxia changed the profile of released inflammatory mediators according to cell type. Interleukin-8 was produced only by post-EMT and basal-like cell lines, regardless of hypoxia. MCP-1 was produced by MDA-MB-435 and -468 cells, whereas IL-6 was present only in MDA-MB-231. IL-2, TNF-α, and NGF production was stimulated by hypoxia in MCF-7 cells. CM from normoxic and hypoxic MDA-MB-231 and MDA-MB-435S cells and hypoxic MCF-7 cells, but not MDA-MB-468, induced NG migration.ConclusionsHypoxia increases migration by the autocrine action of released signal substances in selected luminal and basal-like breast carcinoma cell lines which might explain why anti-angiogenic treatment can worsen clinical outcome in some patients.

【 授权许可】

Unknown   
© Voss et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.