PC-3 prostate carcinoma cells release signal substances that influence the migratory activity of cells in the tumor's microenvironment

Cell Communication and Signaling

Research

Kurt S Zänker¹  Melanie J Voss¹  Bernd Niggemann¹  Frank Entschladen¹ 

[1] Institute of Immunology, ZBAF, Witten/Herdecke University, Stockumer Str. 10, 58448, Witten, Germany;

关键词: Cell Culture Supernatant;    Migratory Activity;    Neutrophil Granulocyte;    Signal Substance;    Prostate Carcinoma Cell;   

DOI  :  10.1186/1478-811X-8-17

 received in 2010-06-10, accepted in 2010-07-13,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundTumor cells interact with the cells of the microenvironment not only by cell-cell-contacts but also by the release of signal substances. These substances are known to induce tumor vascularization, especially under hypoxic conditions, but are also supposed to provoke other processes such as tumor innervation and inflammatory conditions. Inflammation is mediated by two organ systems, the neuroendocrine system and the immune system. Therefore, we investigated the influence of substances released by PC-3 human prostate carcinoma cells on SH-SY5Y neuroblastoma cells as well as neutrophil granulocytes and cytotoxic T lymphocytes, especially with regard to their migratory activity.ResultsPC-3 cells express several cytokines and growth factors including vascular endothelial growth factors, fibroblast growth factors, interleukins and neurotrophic factors. SH-SY5Y cells are impaired in their migratory activity by PC-3 cell culture supernatant, but orientate chemotactically towards the source. Neutrophil granulocytes increase their locomotory activity only in response to cell culture supernantant of hypoxic but not of normoxic PC-3 cells. In contrast, cytotoxic T lymphocytes do not change their migratory activity in response to either culture supernatant, but increase their cytotoxicity, whereas supernatant of normoxic PC-3 cells leads to a stronger increase than that of hypoxic PC-3 cells.ConclusionsPC-3 cells release several signal substances that influence the behavior of the cells in the tumor's microenvironment, whereas no clear pattern towards proinflammatory or immunosuppressive conditions can be seen.

【 授权许可】

Unknown   
© Voss et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.