Establishment of monoclonal HCC cell lines with organ site-specific tropisms

BMC Cancer

Research Article

Ya Cao¹  Jun Tang²  Zhonghua Tao²  Huichuan Sun²  Dongli Liu²  Yang Liu²  Duo Wen²  Lili Dong²  Dongmei Gao²  Jinliang Wan³  Jia Fan⁴  Weizhong Wu⁵ 

[1] Cancer Research Institute, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Central South University, Changsha, Hunan, China;Liver Cancer Institute and Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, 200032, Shanghai, China;Liver Cancer Institute and Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, 200032, Shanghai, China;Department of Oncology, Affiliated Hospital of Binzhou Medical University, 256603, Binzhou, Shandong Province, People’s Republic of China;Liver Cancer Institute and Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, 200032, Shanghai, China;Institute of Biomedical Sciences of Fudan University, 200032, Shanghai, China;Liver Cancer Institute and Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, 200032, Shanghai, China;Liver Cancer Institute and Zhongshan Hospital, Fudan University, 180 Fenglin Road, 200032, Shanghai, China;

关键词: Hepatocellular carcinoma;    Monoclonal cell line;    Metastasis;    Organ-site tropism;    Xenograft model;   

DOI  :  10.1186/s12885-015-1692-0

 received in 2014-09-14, accepted in 2015-10-07,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundOrgan site-specific metastasis is an ominous feature for most poor-prognostic hepatocellular carcinoma (HCC) patients. Cancer cell lines and animal models are indispensable for investigating the molecular mechanisms of organ specific tropism. However, till now, little is known about the drivers in HCC metastatic tropism, and also no effective way has been developed to block the process of tropistic metastasis.MethodsIn this study, we established several monoclonal HCC cell lines from HCCLM3-RFP together with their xenograft models, and then analyzed their metastatic potentials and tropisms using in-vitro and in-vivo assays, and finally elucidated the driving forces of HCC tropistic metastases.ResultsSix monoclonal cell lines with different organ site-specific tropism were established successfully. SPARC, VCAM1 and ANGPTL4 were found positively correlated with the potentials of lung metastasis, while ITGA1 had a positive relation to lymph node metastasis of enterocoelia.ConclusionsBy our powerful platforms, HCC metastatic tropisms in clinic could be easily mimicked and recapitulated for exploring the bilateral interactions between tumor and its microenvironment, elucidating the drivers of HCC metastatic tropisms, and testing anti-cancer effects of newly developed agent in pre-clinical stage.

【 授权许可】

CC BY   
© Wan et al. 2015