Cytotoxicity of purified listeriolysin O on mouse and human leukocytes and leukaemia cells

BMC Biotechnology

Research Article

Jacek Bielecki¹  Radosław Stachowiak¹  Maja Grabowska¹  Katarzyna Roeske¹  Tomasz Jagielski¹  Bożena K Budziszewska²  Marcin Łyżniak³  Grażyna Hoser³  Jerzy Kawiak³ 

[1] Department of Applied Microbiology, Institute of Microbiology, Faculty of Biology, University of Warsaw, I. Miecznikowa 1, 02-096, Warsaw, Poland;Department of Hematology, Institute of Hematology and Transfusion Medicine, I. Gandhi 14, 02-776, Warsaw, Poland;Medical Centre of Postgraduate Education, Marymoncka 99/103, 01-813, Warsaw, Poland;

关键词: Listeriolysin O;    Protein purification;    Cytotoxicity;    Leukocytes;    Leukaemia;   

DOI  :  10.1186/1472-6750-14-77

 received in 2014-02-28, accepted in 2014-08-07,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundListeriolysin O (LLO) is the main virulence factor of Listeria monocytogenes and facilitates the intracellular survival of the pathogen. Some of its characteristics endorse the growing popularity of LLO for use in biotechnology, particularly in the development of novel vaccines. Here, we evaluate the use of LLO to eradicate leukaemia cells.ResultsA purified LLO preparation was obtained by affinity chromatography. The LLO preparation procedure was optimized and purified LLO was tested for optimal conditions of storage including temperature, application of proteinase inhibitors and serum components. We demonstrated the possibility of regulating LLO activity by adjusting cell membrane cholesterol content. The LLO preparation had haemolytic activity and had a cytotoxic effect on the human T-leukaemia Jurkat cell line as well as mouse and human peripheral blood mononuclear cells.ConclusionsLLO has a very potent cytotoxic activity towards human leukocytes. Importantly, the cytotoxic activity was easily regulated in vitro and could be restricted to areas containing malignant cells, raising the possibility of future clinical application of LLO for leukaemia treatment.

【 授权许可】

Unknown   
© Stachowiak et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.