Attenuated RANKL-induced cytotoxicity by Portulaca oleracea ethanol extract enhances RANKL-mediated osteoclastogenesis

BMC Complementary and Alternative Medicine

Research Article

Young-Rae Lee¹  Munkhsoyol Erkhembaatar²  Min Seuk Kim³  Eun-Joo Choi⁴  Hak-Yong Lee⁵  Choong Hun Lee⁶ 

[1] Center for Metabolic Function Regulation (CMFR), Wonkwang University School of Medicine, 570-749, Iksan, Jeonbuk, Republic of Korea;Department of Oral Biochemistry, and Institute of Biomaterial-Implant, College of Dentistry, Wonkwang University, 344-2 Shinyong-dong, 570-749, Iksan, Republic of Korea;Department of Oral Physiology, and Institute of Biomaterial-Implant, College of Dentistry, Wonkwang University, 570-749, Iksan, Jeonbuk, Republic of Korea;Department of Oral Physiology, and Institute of Biomaterial-Implant, College of Dentistry, Wonkwang University, 570-749, Iksan, Jeonbuk, Republic of Korea;Microelectronics and Display Technology, Next Generation Industrial Radiation Technology RIC, Center for PV Human Resource Development, Wonkwang University, 570-749, Iksan, Republic of Korea;Department of Oral and Maxillofacial Surgery, College of Dentistry, Wonkwang University, 570-749, Iksan, Jeonbuk, Republic of Korea;Huvet Inc., 160 Yakchon-ro, 570-979, Iksan, Jeonbuk, Republic of Korea;Microelectronics and Display Technology, Next Generation Industrial Radiation Technology RIC, Center for PV Human Resource Development, Wonkwang University, 570-749, Iksan, Republic of Korea;

关键词: Portulaca oleracea;    Receptor activator of NF-κB ligand;    Bone marrow-derived macrophage;    Osteoclastogenesis;   

DOI  :  10.1186/s12906-015-0770-9

 received in 2015-02-25, accepted in 2015-07-07,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundPortulaca oleracea (PO) has been widely used as traditional medicine because of its pharmacological activities. However, the effects of PO on osteoclasts that modulate bone homeostasis are still elusive.MethodsIn this study, we examined the effects of PO ethanol extract (POEE) on receptor activator of nuclear factor-κB ligand (RANKL)-mediated Ca2+ mobilization, nuclear factor of activated T-cell c1 (NFATc1) amplification, tartrate-resistant acid phosphatase-positive (TRAP+) multinucleated cell (MNC) formation, and cytotoxicity.ResultsOur results demonstrated that POEE suppressed RANKL-induced Ca2+ oscillations by inhibition of Ca2+ release from internal Ca2+ stores, resulting in reduction of NFATc1 amplification. Notably, POEE attenuated RANKL-mediated cytotoxicity and cleavage of polyadenosine 5′-diphosphate-ribose polymerase (PARP), resulted in enhanced formation of TRAP+ MNCs.ConclusionsThese results present in vitro effects of POEE on RANKL-mediated osteoclastogenesis and suggest the possible use of PO in treating bone disorders, such as osteopetrosis.

【 授权许可】

Unknown   
© Erkhembaatar et al. 2015. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.