Attenuated RANKL-induced cytotoxicity by Portulaca oleracea ethanol extract enhances RANKL-mediated osteoclastogenesis

BMC Complementary and Alternative Medicine

Min Seuk Kim⁴  Young-Rae Lee¹  Choong Hun Lee⁴  Hak-Yong Lee²  Eun-Joo Choi³  Munkhsoyol Erkhembaatar⁵ 

[1] Department of Oral Biochemistry, and Institute of Biomaterial-Implant, College of Dentistry, Wonkwang University, 344-2 Shinyong-dong, Iksan 570-749, Republic of Korea;Huvet Inc., 160 Yakchon-ro, Iksan 570-979, Jeonbuk, Republic of Korea;Department of Oral and Maxillofacial Surgery, College of Dentistry, Wonkwang University, Iksan 570-749, Jeonbuk, Republic of Korea;Microelectronics and Display Technology, Next Generation Industrial Radiation Technology RIC, Center for PV Human Resource Development, Wonkwang University, Iksan 570-749, Republic of Korea;Department of Oral Physiology, and Institute of Biomaterial-Implant, College of Dentistry, Wonkwang University, Iksan 570-749, Jeonbuk, Republic of Korea

关键词: Osteoclastogenesis;    Bone marrow-derived macrophage;    Receptor activator of NF-κB ligand;    Portulaca oleracea;   

Others  :  1220037
DOI  :  10.1186/s12906-015-0770-9

 received in 2015-02-25, accepted in 2015-07-07,  发布年份 2015

【 摘 要 】

Background

Portulaca oleracea (PO) has been widely used as traditional medicine because of its pharmacological activities. However, the effects of PO on osteoclasts that modulate bone homeostasis are still elusive.

Methods

In this study, we examined the effects of PO ethanol extract (POEE) on receptor activator of nuclear factor-κB ligand (RANKL)-mediated Ca ²⁺mobilization, nuclear factor of activated T-cell c1 (NFATc1) amplification, tartrate-resistant acid phosphatase-positive (TRAP+) multinucleated cell (MNC) formation, and cytotoxicity.

Results

Our results demonstrated that POEE suppressed RANKL-induced Ca ²⁺oscillations by inhibition of Ca ²⁺release from internal Ca ²⁺stores, resulting in reduction of NFATc1 amplification. Notably, POEE attenuated RANKL-mediated cytotoxicity and cleavage of polyadenosine 5′-diphosphate-ribose polymerase (PARP), resulted in enhanced formation of TRAP+ MNCs.

Conclusions

These results present in vitro effects of POEE on RANKL-mediated osteoclastogenesis and suggest the possible use of PO in treating bone disorders, such as osteopetrosis.

【 授权许可】

   
2015 Erkhembaatar et al.

【 图 表 】

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