Diethyldithiocarbamate inhibits induction of macrophage NO synthase

FEBS Letters

Hauschildt, Sunna²  Mülsch, Alexander¹  Mordvintcev, Peter I.¹  Busse, Rudi¹  Schray-Utz, Beate¹ 

[1] Department of Applied Physiology, University of Freiburg, 7800 Freiburg, Germany;Department of Immunobiology, University of Freiburg, 7800 Freiburg, Germany

关键词: NO synthase;    Transcription;    NFkappaB;    Diethyldithiocarbamate;    Bone marrow-derived macrophage;   

DOI  :  10.1016/0014-5793(93)80111-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We investigated whether sodium diethyldithiocarbamate (DETC), an inhibitor of the nuclear transcription factor kappa B (NFkappaB), modulates induction of NO synthase (NOS) in murine bone marrow-derived macrophages. A short exposure (between 1 and 16 h) of L929-cell medium-preconditioned macrophages to E. coli lipopolysaccharide (LPS) significantly increased the level of NOS mRNA, and elicited NO formation as detected by electron spin resonance spectroscopy and by the release of nitrite. DETC (0.1–1 mM) present during stimulation with LPS prevented the increase in NOS mRNA and the expression of NOS activity. These findings suggest that NFkappaB is involved in the signal transduction pathway linking stimulation of macrophages by LPS with transcription of, the gene encoding inducible NOS.

【 授权许可】

Unknown