Antibacterial activity of crude extracts of some South African medicinal plants against multidrug resistant etiological agents of diarrhoea

BMC Complementary and Alternative Medicine

Research Article

Mary A. Bisi-Johnson¹  Chikwelu L. Obi²  Babatunde B Samuel³  Jacobus N. Eloff³  Anthony I. Okoh⁴ 

[1] Department of Microbiology, Obafemi Awolowo University, Ile-Ife, Nigeria;Department of Medical Microbiology, Walter Sisulu University, 5117, Mthatha, South Africa;SAMRC Microbial Water Quality, Monitoring Centre, University of Fort Hare, Alice, South Africa;Directorate of Academic Affairs, University of Fort Hare, 5700, Alice, South Africa;SAMRC Microbial Water Quality, Monitoring Centre, University of Fort Hare, Alice, South Africa;Phytomedicine Programme, Department of Paraclinical Science, Faculty of Veterinary Science, University of Pretoria, 0110, Onderstepoort, South Africa;SAMRC Microbial Water Quality, Monitoring Centre, University of Fort Hare, Alice, South Africa;

关键词: Medicinal plants;    Diarrhoea;    Antibacterial;    Bioautography;    Phytochemical;   

DOI  :  10.1186/s12906-017-1802-4

 received in 2016-10-14, accepted in 2017-05-22,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThis study evaluated the antibacterial activity of some plants used in folklore medicine to treat diarrhoea in the Eastern Cape Province, South Africa.MethodsThe acetone extracts of Acacia mearnsii De Wild., Aloe arborescens Mill., A. striata Haw., Cyathula uncinulata (Schrad.) Schinz, Eucomis autumnalis (Mill.) Chitt., E. comosa (Houtt.) Wehrh., Hermbstaedtia odorata (Burch. ex Moq.) T.Cooke, Hydnora africana Thunb, Hypoxis latifolia Wight, Pelargonium sidoides DC, Psidium guajava L and Schizocarphus nervosus (Burch.) van der Merwe were screened against Staphylococcus aureus, Escherichia coli, Enterococcus faecalis, multi-resistant Salmonella enterica serovar Isangi, S. typhi, S. enterica serovar Typhimurium, Shigella flexneri type 1b and Sh. sonnei phase II. A qualitative phytochemical screening of the plants extracts was by thin layer chromatography. Plants extracts were screened for antibacterial activity using serial dilution microplate technique and bioautography.ResultsThe TLC fingerprint indicated the presence of terpenoids and flavonoids in the herbs. Most of the tested organisms were sensitive to the crude acetone extracts with minimum inhibitory concentration (MIC) values ranging from 0.018–2.5 mg/mℓ. Extracts of A. striata, C. uncinulata, E. autumnalis and P. guajava were more active against enteropathogens. S. aureus and Sh. flexneri were the most sensitive isolates to the crude extracts but of significance is the antibacterial activity of A. arborescens and P. guajava against a confirmed extended spectrum betalactamase positive S. enterica serovar Typhimurium.ConclusionThe presence of bioactive compounds and the antibacterial activity of some of the selected herbs against multidrug resistant enteric agents corroborate assertions by traditional healers on their efficacies.

【 授权许可】

CC BY   
© The Author(s). 2017