Quantitative trait loci identification, fine mapping and gene expression profiling for ovicidal response to whitebacked planthopper (Sogatella furcifera Horvath) in rice (Oryza sativa L.)

BMC Plant Biology

Research Article

Feng Chen¹  Jiayang Li²  Guosheng Xiong²  Lichao Huang³  Longbiao Guo³  Guangheng Zhang³  Yaolong Yang³  Jiang Hu³  Lan Wang³  Jie Xu³  Yujia Leng³  Qian Qian³  Dali Zeng⁴ 

[1] Department of Plant Sciences, University of Tennessee, 37996, Knoxville, TN, USA;Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China;State Key Lab for Rice Biology, China National Rice Research Institute, 310006, Hangzhou, P. R. China;State Key Lab for Rice Biology, China National Rice Research Institute, 310006, Hangzhou, P. R. China;Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China;

关键词: Fine mapping;    Gene expression profiling;    Ovicidal response;    qWL6;    Sogatella furcifera;    Whitebacked planthopper;   

DOI  :  10.1186/1471-2229-14-145

 received in 2014-01-17, accepted in 2014-05-22,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundThe whitebacked planthopper (WBPH), Sogatella furcifera Horváth, is a serious rice pest in Asia. Ovicidal resistance is a natural rice defense mechanism against WBPH and is characterized by the formation of watery lesions (WLs) and increased egg mortality (EM) at the WBPH oviposition sites.ResultsThis study aimed to understand the genetic and molecular basis of rice ovicidal resistance to WBPH by combining genetic and genomic analyses. First, the ovicidal trait in doubled haploid rice lines derived from a WBPH-resistant cultivar (CJ06) and a WBPH-susceptible cultivar (TN1) were phenotyped based on the necrotic symptoms of the leaf sheaths and EM. Using a constructed molecular linkage map, 19 quantitative trait loci (QTLs) associated with WLs and EM were identified on eight chromosomes. Of them, qWL6 was determined to be a major QTL for WL. Based on chromosome segment substitution lines and a residual heterozygous population, a high-resolution linkage analysis further defined the qWL6 locus to a 122-kb region on chromosome 6, which was annotated to encode 20 candidate genes. We then conducted an Affymetrix microarray analysis to determine the transcript abundance in the CJ06 and TN1 plants. Upon WBPH infestation, 432 genes in CJ06 and 257 genes in TN1 were significantly up-regulated, while 802 genes in CJ06 and 398 genes in TN1 were significantly down-regulated. This suggests that remarkable global changes in gene expression contribute to the ovicidal resistance of rice. Notably, four genes in the 122-kb region of the qWL6 locus were differentially regulated between CJ06 and TN1 in response to the WBPH infestation, suggesting they may be candidate resistance genes.ConclusionsThe information obtained from the fine mapping of qWL6 and the microarray analyses will facilitate the isolation of this important resistance gene and its use in breeding WBPH-resistant rice.

【 授权许可】

Unknown   
© Yang et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.