Characterization and fine mapping of a new dwarf mutant in Brassica napus

BMC Plant Biology

Wei Zhang¹  Xuanming Liu²  Jindong Yan³  Fujiang Xiang³  Piao Yang³  Xiaoying Zhao³  Xinmei Li³  Ming Zhong³  Xin Li⁴  Caiyan Chen⁵  Donghai Mao⁵ 

[1] College of Agronnomy, Hunan Agricultural University, 410128, Changsha, China;College of Biology, Hunan Hybrid Rape Engineering and Technology Research Center, Hunan University, 410082, Changsha, China;College of Biology, Hunan Hybrid Rape Engineering and Technology Research Center, Hunan University, 410082, Changsha, China;Shenzhen Institute, Hunan University, 518057, Shenzhen, China;College of Biology, Hunan Hybrid Rape Engineering and Technology Research Center, Hunan University, 410082, Changsha, China;Shenzhen Institute, Hunan University, 518057, Shenzhen, China;Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, 410125, Changsha, China;Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, 410125, Changsha, China;

关键词: Brassica napus;    Dwarf;    Grain yield;    BSA;    Fine mapping;   

DOI  :  10.1186/s12870-021-02885-y

来源: Springer

【 摘 要 】

BackgroundPlant height is an important plant characteristic closely related to yield performance of many crops. Reasonable reduction of plant height of crops is beneficial for improving yield and enhancing lodging resistance.ResultsIn the present study, we described the Brassica napus dwarf mutant bnd2 that was isolated using ethyl methanesulfonate (EMS) mutagenesis. Compared to wild type (WT), bnd2 exhibited reduced height and shorter hypocotyl and petiole leaves. By crossing the bnd2 mutant with the WT strain, we found that the ratio of the mutant to the WT in the F2 population was close to 1:3, indicating that bnd2 is a recessive mutation of a single locus. Following bulked segregant analysis (BSA) by resequencing, BND2 was found to be located in the 13.77–18.08 Mb interval of chromosome A08, with a length of 4.31 Mb. After fine mapping with single nucleotide polymorphism (SNP) and insertion/deletion (InDel) markers, the gene was narrowed to a 140-Kb interval ranging from 15.62 Mb to 15.76 Mb. According to reference genome annotation, there were 27 genes in the interval, of which BnaA08g20960D had an SNP type variation in the intron between the mutant and its parent, which may be the candidate gene corresponding to BND2. The hybrid line derived from a cross between the mutant bnd2 and the commercial cultivar L329 had similar plant height but higher grain yield compared to the commercial cultivar, suggesting that the allele bnd2 is beneficial for hybrid breeding of lodging resistant and high yield rapeseed.ConclusionIn this study, we identified a novel dwarf mutant of rapeseed with a new locus, which may be useful for functional analyses of genetic mechanisms of plant architecture and grain yield in rapeseed.

【 授权许可】

CC BY