Molecular cytogenetic identification of a novel dwarf wheat line with introgressed Thinopyrum ponticum chromatin

Journal of biosciences

Yinguang Bao²  Xingfeng Li1²  Honggang Wang²  Q I Zheng¹  Guiling Chen²  Shubing Liu² 

[1] State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China$$;State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Tai'an Subcenter of the National Wheat Improvement Center, Agronomy College, Shandong Agricultural University, Taian 271018, Shandong, China$$

关键词: Dwarf;    introgression line;    Thinopyrum ponticum;    wheat;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

Novel dwarfing germplasms and dwarfing genes are valuable for the wheat breeding. A novel semi-dwarf line, 31505-1, with reduced height compared with its common wheat parent, was derived from a cross between common wheat and Thinopyrum ponticum. Cytological studies demonstrated that 31505-1 contained 42 chromosomes and formed 21 bivalents at meiotic metaphase I. Genomic in situ hybridization (GISH) analysis showed that 31505-1 had no large Th. ponticum chromosome fragments. Fluorescence in situ hybridization (FISH) results revealed the absence of a pAs1 hybridization band on 2DL chromosome of 31505-1. Two SSR markers (Xwmc41 and Xcfd168) and two STS markers (Xmag4059 and Xmag3596), which were located on 2D chromosome, amplified unique bands of Th. Ponticum in 31505-1. These revealed presence of an introgressed Th. ponticum segment in 2DL chromosome of dwarf line 31505-1, although the alien segment could not be detected by GISH.

【 授权许可】

Unknown