The rice pds1 locus genetically interacts with partner to cause panicle exsertion defects and ectopic tillers in spikelets

BMC Plant Biology

Xuan Wang¹  Baoxiang Qin¹  Peilong Jia¹  Yindi Zeng²  Qigui Jiang²  Jijing Luo²  Baiyang Yu²  Siyuan Lu²  Weijian Cen³  Zhongquan Cai⁴ 

[1] 0000 0001 2254 5798, grid.256609.e, Agriculture College, Guangxi University, 530004, Nanning, China;0000 0001 2254 5798, grid.256609.e, College of Life Science and technology (State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources), Guangxi University, 530004, Nanning, China;0000 0001 2254 5798, grid.256609.e, College of Life Science and technology (State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources), Guangxi University, 530004, Nanning, China;0000 0001 2254 5798, grid.256609.e, Agriculture College, Guangxi University, 530004, Nanning, China;0000 0001 2254 5798, grid.256609.e, College of Life Science and technology (State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources), Guangxi University, 530004, Nanning, China;0000 0001 2254 5798, grid.256609.e, Institute of New Rural Development, Guangxi University, 530004, Nanning, China;0000 0001 2254 5798, grid.256609.e, Agriculture College, Guangxi University, 530004, Nanning, China;

关键词: Rice;    Sheathed panicle;    Aberrant spikelets;    High-order tillering;    Panicle tillering;    Fine mapping;    Genetic interaction;   

DOI  :  10.1186/s12870-019-1805-z

来源: publisher

【 摘 要 】

BackgroundRice (Oryza sativa L.) is a staple food crop worldwide. Its yield and quality are affected by its tillering pattern and spikelet development. Although many genes involved in the vegetative and reproductive development of rice have been characterized in previous studies, the genetic mechanisms that control axillary tillering, spikelet development, and panicle exsertion remain incompletely understood.ResultsHere, we characterized a novel rice recombinant inbred line (RIL), panicle exsertion defect and aberrant spikelet (pds). It was derived from a cross between two indica varieties, S142 and 430. Intriguingly, no abnormal phenotypes were observed in the parents of pds. This RIL exhibited sheathed panicles at heading stage. Still, a small number of tillers in pds plants were fully exserted from the flag leaves. Elongated sterile lemmas and rudimentary glumes (occurred occasionally) were observed in the spikelets of the exserted panicles and were transformed into palea/lemma-like structures. Furthermore, more interestingly, tillers occasionally grew from the axils of the elongated rudimentary glumes. Via genetic linkage analysis, we found that the abnormal phenotype of pds manifesting as genetic incompatibility or hybrid weakness was caused by genetic interaction between a recessive locus, pds1, which was derived from S142 and mapped to chromosome 8, and a locus pds2, which not yet mapped from 430. We fine-mapped pds1 to an approximately 55-kb interval delimited by the markers pds-4 and 8 M3.51. Six RGAP-annotated ORFs were included in this genomic region. qPCR analysis revealed that Loc_Os080595 might be the target of pds1 locus, and G1 gene might be involved in the genetic mechanism underlying the pds phenotype.ConclusionsIn this study, histological and genetic analyses revealed that the pyramided pds loci resulted in genetic incompatibility or hybrid weakness in rice might be caused by a genetic interaction between pds loci derived from different rice varieties. Further isolation of pds1 and its interactor pds2, would provide new insight into the molecular regulation of grass inflorescence development and exsertion, and the evolution history of the extant rice.

【 授权许可】

CC BY