Expression of an osmotin-like protein from Solanum nigrumconfers drought tolerance in transgenic soybean

BMC Plant Biology

Research Article

Giovani Greigh de Brito¹  Regina Maria Santos de Amorim²  Magda Aparecida Beneventi²  Paulo Fernando Bertagnolli³  Maria Eugênia Lisei de Sá⁴  Magnólia de Araújo Campos⁵  Ricardo Luís Mayer Weber⁶  Rogério Margis⁶  Beatriz Wiebke-Strohm⁶  Ciliana Rechenmacher⁶  Christian Bredemeier⁶  Márcia Margis-Pinheiro⁶  Maria Helena Bodanese-Zanettini⁷  Maria Fátima Grossi-de-Sa⁸ 

[1] Embrapa Clima Temperado, 96010-971, Pelotas, RS, Brazil;Embrapa Recursos Genéticos e Biotecnologia, 70770-917, Brasilia, DF, Brazil;Embrapa Trigo, 99001-970, Passo Fundo, RS, Brazil;Empresa de Pesquisa Agropecuária de Minas Gerais, 38001-970, Uberaba, MG, Brazil;Embrapa Recursos Genéticos e Biotecnologia, 70770-917, Brasilia, DF, Brazil;Universidade Federal de Campina Grande, 58175-000, Cuité, PB, Brazil;Universidade Federal do Rio Grande do Sul, 91501-970, Porto Alegre, RS, Brazil;Universidade Federal do Rio Grande do Sul, 91501-970, Porto Alegre, RS, Brazil;Departamento de Genética, Instituto de Biociências, Av, Bento Gonçalves, 9500, CP 15053, 91501-970, Porto Alegre, RS, Brazil;Universidade Federal do Rio Grande do Sul, 91501-970, Porto Alegre, RS, Brazil;Embrapa Recursos Genéticos e Biotecnologia, 70770-917, Brasilia, DF, Brazil;

关键词: Abiotic stress;    Bombardment;    Drought tolerance;    Genetic transformation;    Glycine max;    Osmotin;    Water deficit;   

DOI  :  10.1186/s12870-014-0343-y

 received in 2014-05-26, accepted in 2014-11-20,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundDrought is by far the most important environmental factor contributing to yield losses in crops, including soybeans [Glycine max (L.) Merr.]. To address this problem, a gene that encodes an osmotin-like protein isolated from Solanum nigrum var. americanum (SnOLP) driven by the UBQ3 promoter from Arabidopsis thaliana was transferred into the soybean genome by particle bombardment.ResultsTwo independently transformed soybean lines expressing SnOLP were produced. Segregation analyses indicated single-locus insertions for both lines. qPCR analysis suggested a single insertion of SnOLP in the genomes of both transgenic lines, but one copy of the hpt gene was inserted in the first line and two in the second line. Transgenic plants exhibited no remarkable phenotypic alterations in the seven analyzed generations. When subjected to water deficit, transgenic plants performed better than the control ones. Leaf physiological measurements revealed that transgenic soybean plants maintained higher leaf water potential at predawn, higher net CO2 assimilation rate, higher stomatal conductance and higher transpiration rate than non-transgenic plants. Grain production and 100-grain weight were affected by water supply. Decrease in grain productivity and 100-grain weight were observed for both transgenic and non-transgenic plants under water deficit; however, it was more pronounced for non-transgenic plants. Moreover, transgenic lines showed significantly higher 100-grain weight than non-transgenic plants under water shortage.ConclusionsThis is the first report showing that expression of SnOLP in transgenic soybeans improved physiological responses and yield components of plants when subjected to water deficit, highlighting the potential of this gene for biotechnological applications.

【 授权许可】

Unknown   
© Weber et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.