Molecular Characterization of Rice OsbZIP66 Transcription Factor for Drought Tolerance

OsbZIP66 Transcription Factor;Drought tolerance;631

국제농업기술대학원 국제농업기술학과 ;

University:서울대학교 대학원

关键词: OsbZIP66 Transcription Factor;    Drought tolerance;    631;   

Others  :  http://s-space.snu.ac.kr/bitstream/10371/127129/1/000000141157.pdf

美国|英语

来源: Seoul National University Open Repository

【 摘 要 】

The basic leucine zipper (bZIP) TF family is one of the largest plant-specific TF families and particularly, group-A Arabiodpsis bZIP TFs are known to be involved in abiotic stress responses. In this study, we isolated and functionally characterized OsbZIP66 which belongs to the group-A AtbZIP TFs. To investigate the response of OsbZIP66 to abiotic stresses, quantitative RT-PCR was performed and resulted that the transcriptional expression of OsbZIP66 is induced by drought stress and ABA. Examination of putative OsbZIP66 promoter revealed that there are several ABA-responsive elements (ABREs), suggesting that OsbZIP66 regulates drought stress in an ABA-dependent manner. Further phenotypic and chlorophyll fluorescence analysis proved that overexpression of OsbZIP66 in both whole plant body and specifically in root enhanced drought tolerance in rice. These results indicate that the drought tolerance of rice has positive correlation with the expression level of OsbZIP66 and the overexpression of OsbZIP66 in roots is important for drought tolerance. Taken together, OsbZIP66 has a high potential to improve drought tolerance in rice.