Establishment of Regeneration System of Potato Variety Xuanshu 2

2018 4th International Conference on Environmental Science and Material Application

生态环境科学;材料科学

Zheng, Hao^1 ; Liang, Sha^1 ; Zheng, Aihong^1 ; Yuan, Qiao^1 ; Ma, Jie^2 ; Zhou, Ping^2 ; Sun, Bo^1

College of Horticulture, Sichuan Agricultural University, Chengdu

611130, China^1

Bijie Institute of Agricultural Science, Bijie

551700, China^2

关键词: Callus induction;    Cultivated potato;    Differentiation rate;    Genetic transformation;    Optimal combination;    Plant material;    Potato varieties;    Regeneration system;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/252/2/022019/pdf
DOI  :  10.1088/1755-1315/252/2/022019

来源: IOP

【 摘 要 】

In this study, the cultivated potato variety Xuanshu 2 was used as plant material, and MS was used as the basic medium, the in vitro regeneration culture of stem segments was carried out to study the optimal combination of phytohormone concentrations for callus induction and bud differentiation. The results indicated that the optimal medium for callus induction in stem segments was MS + 2.0 mg•L-1 6-BA + 0.2 mg•L-1 NAA, with 100% induction rate. The optimal medium for bud differentiation was MS + 2.0 mg•L-1 6-BA + 2.0 mg•L-1 ZT + 0.5 mg•L-1 GA3, with 88% differentiation rate. In this study, the regeneration system of potato variety Xuanshu 2 was established, laying the foundation for the subsequent genetic transformation research of this variety.