Protective effects of hydrogen enriched saline on liver ischemia reperfusion injury by reducing oxidative stress and HMGB1 release

BMC Gastroenterology

Research Article

Daqing Ma¹  Marcela P Vizcaychipi¹  Dafydd M Lloyd¹  Michael G Irwin²  Yantao Liu³  Weifeng Yu³  Kunming Tao³  Liqun Yang³  Xuejun Sun⁴ 

[1]Anaesthetics, Pain Medicine & Intensive Care, Department of Surgery and Cancer, Imperial College London, Chelsea and Westminster Hospital, SW10 9NH, London, UK

[2]Department of Anaesthesiology, University of Hong Kong, Queen Mary Hospital, K424, Hong Kong, China

[3]Department of Anesthesia & Intensive Care, Eastern Hepatobiliary Surgical hospital, the Second Military Medical University, 200433, Shanghai, China

[4]Department of Diving Medicine, Faculty of Naval Medicine, the Second Military Medical University, 200433, Shanghai, China

关键词: Hepatology;    Ischemia/reperfusion injury;    Inflammatory mediators;    Oxidant stress;   

DOI  :  10.1186/1471-230X-14-12

 received in 2013-01-02, accepted in 2013-12-30,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundThe nuclear protein high-mobility group box 1 (HMGB1) is a key trigger for the inflammatory reaction during liver ischemia reperfusion injury (IRI). Hydrogen treatment was recently associated with down-regulation of the expression of HMGB1 and pro-inflammatory cytokines during sepsis and myocardial IRI, but it is not known whether hydrogen has an effect on HMGB1 in liver IRI.MethodsA rat model of 60 minutes 70% partial liver ischemia reperfusion injury was used. Hydrogen enriched saline (2.5, 5 or 10 ml/kg) was injected intraperitoneally 10 minutes before hepatic reperfusion. Liver injury was assessed by serum alanine aminotransferase (ALT) enzyme levels and histological changes. We also measured malondialdehyde (MDA), hydroxynonenal (HNE) and 8-hydroxy-guanosine (8-OH-G) levels as markers of the peroxidation injury induced by reactive oxygen species (ROS). In addition, pro-inflammatory cytokines including TNF-α and IL-6, and high mobility group box B1 protein (HMGB1) were measured as markers of post ischemia-reperfusion inflammation.ResultsHydrogen enriched saline treatment significantly attenuated the severity of liver injury induced by ischemia-reperfusion. The treatment group showed reduced serum ALT activity and markers of lipid peroxidation and post ischemia reperfusion histological changes were reduced. Hydrogen enriched saline treatment inhibited HMGB1 expression and release, reflecting a reduced local and systemic inflammatory response to hepatic ischemia reperfusion.ConclusionThese results suggest that, in our model, hydrogen enriched saline treatment is protective against liver ischemia-reperfusion injury. This effect may be mediated by both the anti-oxidative and anti-inflammatory effects of the solution.

【 授权许可】

Unknown   
© Liu et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.