Protective effects of hydrogen enriched saline on liver ischemia reperfusion injury by reducing oxidative stress and HMGB1 release

BMC Gastroenterology

Weifeng Yu¹  Daqing Ma³  Michael G Irwin²  Xuejun Sun⁴  Dafydd M Lloyd³  Marcela P Vizcaychipi³  Kunming Tao¹  Liqun Yang¹  Yantao Liu¹ 

[1]Department of Anesthesia & Intensive Care, Eastern Hepatobiliary Surgical hospital, the Second Military Medical University, Shanghai 200433, China

[2]Department of Anaesthesiology, University of Hong Kong, Queen Mary Hospital, Hong Kong K424, China

[3]Anaesthetics, Pain Medicine & Intensive Care, Department of Surgery and Cancer, Imperial College London, Chelsea and Westminster Hospital, London SW10 9NH, UK

[4]Department of Diving Medicine, Faculty of Naval Medicine, the Second Military Medical University, Shanghai 200433, China

关键词: Oxidant stress;    Inflammatory mediators;    Ischemia/reperfusion injury;    Hepatology;   

Others  :  856698
DOI  :  10.1186/1471-230X-14-12

 received in 2013-01-02, accepted in 2013-12-30,  发布年份 2014

【 摘 要 】

Background

The nuclear protein high-mobility group box 1 (HMGB1) is a key trigger for the inflammatory reaction during liver ischemia reperfusion injury (IRI). Hydrogen treatment was recently associated with down-regulation of the expression of HMGB1 and pro-inflammatory cytokines during sepsis and myocardial IRI, but it is not known whether hydrogen has an effect on HMGB1 in liver IRI.

Methods

A rat model of 60 minutes 70% partial liver ischemia reperfusion injury was used. Hydrogen enriched saline (2.5, 5 or 10 ml/kg) was injected intraperitoneally 10 minutes before hepatic reperfusion. Liver injury was assessed by serum alanine aminotransferase (ALT) enzyme levels and histological changes. We also measured malondialdehyde (MDA), hydroxynonenal (HNE) and 8-hydroxy-guanosine (8-OH-G) levels as markers of the peroxidation injury induced by reactive oxygen species (ROS). In addition, pro-inflammatory cytokines including TNF-α and IL-6, and high mobility group box B1 protein (HMGB1) were measured as markers of post ischemia-reperfusion inflammation.

Results

Hydrogen enriched saline treatment significantly attenuated the severity of liver injury induced by ischemia-reperfusion. The treatment group showed reduced serum ALT activity and markers of lipid peroxidation and post ischemia reperfusion histological changes were reduced. Hydrogen enriched saline treatment inhibited HMGB1 expression and release, reflecting a reduced local and systemic inflammatory response to hepatic ischemia reperfusion.

Conclusion

These results suggest that, in our model, hydrogen enriched saline treatment is protective against liver ischemia-reperfusion injury. This effect may be mediated by both the anti-oxidative and anti-inflammatory effects of the solution.

【 授权许可】

   
2014 Liu et al.; licensee BioMed Central Ltd.

【 图 表 】

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