Large-scale cell production of stem cells for clinical application using the automated cell processing machine

BMC Biotechnology

Research Article

Tomoya Kitani¹  Satoshi Gojo²  Daisuke Kami²  Mayu Yamazaki-Inoue³  Kahori Minami³  Akihiro Umezawa³  Yoko Itakura⁴  Masashi Toyoda⁴  Takashi Sakurai⁵  Keizo Watakabe⁵ 

[1] Department of Cardiovascular Medicine, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kawaramachi-Hirokoji, 602-8566, Kamigyo-ku, Kyoto, Japan;Department of Regenerative Medicine, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kawaramachi-Hirokoji, 602-8566, Kamigyo-ku, Kyoto, Japan;Department of Reproductive Biology and Pathology, National Center for Child Health and Development, 2-10-1 Okura, 157-8535, Setagaya-ku, Tokyo, Japan;Department of Vascular Medicine, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakae-cho, 173-0015, Itabashi-ku, Tokyo, Japan;System Technology Development Center, Kawasaki Heavy Industries, Ltd, 3-1-1 Higashi Kawasaki-cho, 650-8670, Chuo-ku, Kobe, Japan;

关键词: Automated cell culture system;    Cell transplantation;    Stem cells;    Clinical trial;    Cell processing facility;   

DOI  :  10.1186/1472-6750-13-102

 received in 2013-05-07, accepted in 2013-11-12,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundCell-based regeneration therapies have great potential for application in new areas in clinical medicine, although some obstacles still remain to be overcome for a wide range of clinical applications. One major impediment is the difficulty in large-scale production of cells of interest with reproducibility. Current protocols of cell therapy require a time-consuming and laborious manual process. To solve this problem, we focused on the robotics of an automated and high-throughput cell culture system. Automated robotic cultivation of stem or progenitor cells in clinical trials has not been reported till date. The system AutoCulture® used in this study can automatically replace the culture medium, centrifuge cells, split cells, and take photographs for morphological assessment. We examined the feasibility of this system in a clinical setting.ResultsWe observed similar characteristics by both the culture methods in terms of the growth rate, gene expression profile, cell surface profile by fluorescence-activated cell sorting, surface glycan profile, and genomic DNA stability. These results indicate that AutoCulture® is a feasible method for the cultivation of human cells for regenerative medicine.ConclusionsAn automated cell-processing machine will play important roles in cell therapy and have widespread use from application in multicenter trials to provision of off-the-shelf cell products.

【 授权许可】

Unknown   
© Kami et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.