Euphorbia tirucalli modulates gene expression in larynx squamous cell carcinoma

BMC Complementary and Alternative Medicine

Research Article

Flávia Cristina Rodrigues-Lisoni¹  Gabriela Bueno Franco-Salla²  Laila Toniol Cardin²  Janesly Prates²  Sonia Maria Oliani²  Wilson Araújo da Silva Jr³  Anemari Ramos Dinarte dos Santos³  Eloiza Helena Tajara⁴  Bianca Rodrigues da Cunha⁴ 

[1]Department of Biology and Animal Science, Faculty of Engineering of Ilha Solteira - FEIS/UNESP, Av. Brasil, 56, CEP: 15385-000, Ilha Solteira, São Paulo, Brazil

[2]Department of Biology, Institute of Biosciences, Letters and Science - IBILCE/UNESP, São José do Rio Preto, SP, Brazil

[3]Department of Clinical Medical, Foundation Blood Center of Ribeirão Preto, Faculty of Medicine of Ribeirão Preto, University of São Paulo - FCFRP/USP, Ribeirão Preto, SP, Brazil

[4]Department of Molecular Biology, Faculty of Medicine of São José do Rio Preto - FAMERP, São José do Rio Preto, SP, Brazil

关键词: Differential gene expression;    Cell culture;    Subtractive hybridization;    Quantitative PCR;   

DOI  :  10.1186/s12906-016-1115-z

 received in 2015-09-02, accepted in 2016-05-13,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundSome plants had been used in the treatment of cancer and one of these has attracted scientific interest, the Euphorbia tirucalli (E. tirucalli), used in the treatment of asthma, ulcers, warts has active components with activities scientifically proven as antimutagenic, anti-inflammatory and anticancer.MethodsWe evaluate the influence of the antitumoral fraction of the E. tirucalli latex in the larynx squamous cell carcinoma (Hep-2), on the morphology, cell proliferation and gene expression. The Hep-2 cells were cultivated in complete medium (MEM 10 %) and treated with E. tirucalli latex for 1, 3, 5 and 7 days. After statistically analyzing the proliferation of the tested cells, the cells were cultivated again for RNA extraction and the Rapid Subtractive Hybridization (RaSH) technique was used to identify genes with altered expression. The genes found using the RaSH technique were analyzed by Gene Ontology (GO) using Ingenuity Systems.ResultsThe five genes found to have differential expression were validated by real-time quantitative PCR. Though treatment with E. tirucalli latex did not change the cell morphology in comparison to control samples, but the cell growth was significantly decreased. The RaSH showed change in the expression of some genes, including ANXA1, TCEA1, NGFRAP1, ITPR1 and CD55, which are associated with inflammatory response, transcriptional regulation, apoptosis, calcium ion transport regulation and complement system, respectively. The E. tirucalli latex treatment down-regulated ITPR1 and up-regulated ANXA1 and CD55 genes, and was validated by real-time quantitative PCR.ConclusionsThe data indicate the involvement of E. tirucalli latex in the altered expression of genes involved in tumorigenic processes, which could potentially be applied as a therapeutic indicator of larynx cancer.

【 授权许可】

CC BY   
© Franco-Salla et al. 2016