Isolation of a cDNA clone encoding a novel membrane protein expressed in lymphocytes

FEBS Letters

Mizuno, Ryuzo²  Shimizu, Yoshifumi²  Sugiyama, Haruo²  Oka, Yoshihiro²  Kishimoto, Tadamitsu²  Sakoda, Saburo¹  Ogawa, Hiroyasu² 

[1] Department of Neurology, Osaka University Medical School, 2-2, Yamada-Oka, Suita City, Osaka 565, Japan;Department of Medicine III, Osaka University Medical School, 2-2, Yamada-Oka, Suita City, Osaka 565, Japan

关键词: Lymphocyte-specific cDNA;    Embryonic stage-specific cDNA;    Subtractive hybridization;    Single stranded phagemid;   

DOI  :  10.1016/0014-5793(94)01164-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

By subtractive hybridization using single-stranded phagemids with directional inserts, we isolated a mouse cDNA clone, LSM-1, from temperature-sensitive Abelson virus-tranformed immature B cells whose differentiation was being induced after the shift from the permissive (35°C) to the non-permissive temperature (39°C). LSM-1, which encodes an as yet unknown peptide of 197 amino acids, has a putative signal sequence and a trans-membrane region, and is expressed in B- and T-cell lines, in spleen, thymus, and bone marrow of adult mice, and in embryos.

【 授权许可】

Unknown