Isolation of tissue‐type plasminogen activator, cathepsin H, and non‐specific cross‐reacting antigen from SK‐PC‐1 pancreas cancer cells using subtractive hybridization

FEBS Letters

Paciucci, Rosanna¹  Torà, Montserrat¹  Real, Francisco X.¹  García de Herreros, Antonio¹  Navarro, Estanis¹  Berrozpe, Georgina¹ 

[1] Departament d'Immunologia, Institut Municipal d'Investigació Mèdica (IMIM), c/Dr. Aiguader 80, Barcelona 08003, Spain

关键词: Pancreas cancer;    Tissue-type plasminogen activator;    Thymosin β4;    Non-specific cross-reacting antigen;    Subtractive hybridization;    Tβ4;    thymosin β4;    tPA;    tissue type plasminogen activator;    NCA;    non-specific cross-reacting antigen;    MTS-1;    multiple tumor suppressor 1 gene;    HMGl;    high mobility group 1;    CEA;    carcinoembryonic antigen;    CK;    cytokeratin;   

DOI  :  10.1016/0014-5793(96)00352-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have used subtractive hybridization to isolate cDNAs overexpressed in SK-PC-1 pancreas cancer cells. Fortyfive independent clones corresponding to 11 genes were identified. Their expression in cultured pancreas cancer cells, normal pancreas tissue, and normal exocrine pancreas cultures was examined by Northern blotting. cDNA clones can be grouped into two broad categories: (1) those corresponding to genes expressed at high levels both in tumor cell lines and in primary cultures of normal pancreas, but not in normal tissue (i.e. thymosin β4³,, cytokeratin 18, β-actin, pyruvate kinase and mitochondrial genes); and (2) those corresponding to genes expressed at high levels in pancreas cancer cultures but not in normal pancreas tissue or cultured cells (i.e. tissue-type plasminogen activator and cathepsin H). The overexpression of these proteases in pancreas cancers suggests that they play a role in the aggressive biological behavior of this tumor.

【 授权许可】

Unknown