Selection of down‐regulated sequences along the monocytic differentiation of leukemic HL60 cells

FEBS Letters

Bezian, J.H¹  Bonnet, J¹  Najeme, F¹  Herblot, S¹  de Miguel, C²  Vekris, A³  Rouzaut, A² 

[1] Laboratoire d'Immunologie Moléculaire, Université de Bordeaux II, Zone Nord bat. 1b, 146 Rue Léo Saignat, 33076 Bordeaux Cedex, France;Departement de Biochemica, Universidad de Navarra, Appartado 177, 31080 Pampelona, Spain;Laboratoire de Pathologie Moléculaire et de Thérapie Génique, Université de Bordeaux II, Zone Nord bat. 1b, 146 Rue Léo Saignat, 33076 Bordeaux Cedex, France

关键词: Subtractive hybridization;    Monocytic differentiation;    Leukemia;    α-Tubulin;    Ribosomal protein;    TaxREB;    RDA;    representational difference analysis;    TPA;    12-O-tetradecanoylphorbol-13-acetate;    MPO;    myeloperoxidase;    CREB;    cAMP-responsive element-binding protein;    UP;    universal primer;    FUSE-BP;    far upstream binding protein;    RBP;    ribosomal protein;   

DOI  :  10.1016/S0014-5793(97)00967-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In order to dissect the molecular mechanisms of monocytic differentiation we have developed a subtractive hybridisation method based on a simplified `representational difference analysis'. We have selected 16 sequences and confirmed their down-regulation along the TPA-induced monocytic differentiation of HL60 cells. Among these sequences we have identified the α-tubulin, the TaxREB protein and two ribosomal protein sequences which had not been previously described as differentially expressed. These results add to our knowledge about the molecules implicated along the monocytic differentiation and growth arrest of leukemic cells and provide a first step in the study of their respective roles.

【 授权许可】

Unknown