Differentiation‐associated expression of prostaglandin G/H synthase in monocytic cells

FEBS Letters

Kaever, Volkhard¹  Resch, Klaus¹  Goppelt-Struebe, Margarete³  Hoff, Torsten¹  DeWitt, David² 

[1] Institute of Molecular Pharmacology,, Medical School Hannover, Konstanty-Gutschow-Str. 8, W-3000 Hannover 61, Germany;Biochemistry Building, Room 510, Department of Biochemistry, Michigan State University, East Lansing, MI 48824-1319, USA;Nephrology Laboratory, Department of Medicine IV, University of Erlangen-Nürnberg, Loschgestrasse 8 112, W-8520 Erlangen, Germany

关键词: Prostaglandin G/H synthase;    Prostanoid;    Monocytic differentiation;    Corticosteroid;    HEL;    human erythroleukemia;    HUVE;    human umbilical vein endothelial;    IL-1β;    interleukin-1β;    LPS;    lipopolysaccharide;    PG;    prostaglandin;    PGHS;    prostaglandin G/H synthase;    TPA;    12-O-tetradecanoylphorbol-13-acetate;    Tx;    thromboxane;   

DOI  :  10.1016/0014-5793(93)81653-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Significant progress in the investigation of the regulation of prostanoid formation has recently been made by cloning a second gene coding for prostaglandin G/H synthase (PGHS; EC 1.14.99.1). In this study we examined the expression of the two PGHS isoforms during phorbol ester induced monocytic differentiation of human myeloid leukemia cells (U937). Murine and ovine PGHS-1 probes hybridized to 2.8- and 5.5-kb mRNA species, whereas the murine PGHS-2 probe hybridized to a 5.3-kb species. Western blot analysis using antisera to mouse PGHS-1 and to a synthetic peptide derived from a mouse PGHS-2-specific region revealed a band of 70 kDa for PGHS-1 and a doublet of about 85 kDa for PGHS-2. Unlike PGHS-2, which was not expressed in U937 control cells, both PGHS-1 protein and mRNA were detected in untreated U937 cells. TPA strongly induced PGHS-2 protein and also increased the amount of PGHS-1 protein. Correspondingly, a marked induction of PGHS-2 mRNA was found, but virtually no change in the expression of the PGHS-1 2.8-kb mRNA occurred. The induction of both PGHS isoforms turned out to be dexamethasone-sensitive. The suppression of PGHS-2 induction was more pronounced. These results suggest that both PGHS-1 and to a larger extent PGHS-2 contribute to the upregulation of prostanoid synthesis during monocytic differentiation.

【 授权许可】

Unknown