Massive deregulation of miRNAs from nuclear reprogramming errors during trophoblast differentiation for placentogenesis in cloned pregnancy

BMC Genomics

Research Article

Gregor Kirfel¹  Franca Rings²  Jasim Uddin²  Michael Hoelker²  Ernst Tholen²  Eva Held²  Dessie Salilew-Wondim²  Maren J Pröll²  Dawit Tesfaye²  Christian Looft²  Karl Schellander²  Md Munir Hossain³ 

[1]Institute for Cell Biology, University of Bonn, Ulrich-Haberland-Strasse 61a, 53121, Bonn, Germany

[2]Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, Endenicher Allee 15, 53115, Bonn, Germany

[3]Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, Endenicher Allee 15, 53115, Bonn, Germany

[4]Animal Breeding & Genetics, Bangladesh Agricultural University, 2202, Mymensingh, Bangladesh

关键词: miRNAs;    IVP;    SCNT;    Reprogramming;    Placenta;    Trophoblast;    Differentiation;    Bovine;   

DOI  :  10.1186/1471-2164-15-43

 received in 2013-05-08, accepted in 2014-01-08,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundLow efficiency of Somatic Cell Nuclear Transfer (NT) has been widely addressed with high incidence of placental abnormalities due to genetic and epigenetic modifications. MiRNAs are shown to be major regulators of such modifications. The present study has been carried out to identify the expression patterns of 377 miRNAs, their functional associations and mechanism of regulation in bovine placentas derived from artificial insemination (AI), in vitro production (IVP) and NT pregnancies.ResultsThis study reveals a massive deregulation of miRNAs as chromosomal cluster or miRNA families without sex-linkage in NT and in-vitro derived IVP placentas. Cell specific localization miRNAs in blastocysts and expression profiling of embryos and placentas at different developmental stages identified that the major deregulation of miRNAs exhibited in placentas at day 50 of pregnancies is found to be less dependent on global DNA methylation, rather than on aberrant miRNA biogenesis molecules. Among them, aberrant AGO2 expression due to hypermethylation of its promoter was evident. Along with other factors, aberrant AGO2 expression was observed to be associated with multiple defects in trophoblast differentiation through deregulation of miRNAs mediated mechanisms.ConclusionThese aberrant miRNA activities might be associated with genetic and epigenetic modifications in abnormal placentogenesis due to maldifferentiation of early trophoblast cell lineage in NT and IVP pregnancies. This study provides the first insight into genome wide miRNA expression, their role in regulation of trophoblast differentiation as well as abnormal placental development in Somatic Cell Nuclear Transfer pregnancies to pave the way to improve the efficiency of cloning by nuclear transfer.

【 授权许可】

Unknown   
© Hossain et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.