Reproductive Seasonality Affects In Vitro Embryo Production Outcomes in Adult Goats

Animals

JoannaM.G. Souza-Fabjan¹  LucasF.L. Correia¹  RibrioI.T.P. Batista¹  Pascal Mermillod²  VicenteJ.F. Freitas³  Yann Locatelli⁴ 

[1] Faculdade de Veterinária, Universidade Federal Fluminense, Niterói, RJ 24230-340, Brazil;Institut National de Recherche Pour L’agriculture, L’alimentation et L’environnement (INRAE), Physiologie de la Reproduction et des Comportements, 37380 Nouzilly, France;Laboratório de Fisiologia e Controle da Reprodução, Universidade Estadual do Ceará, Fortaleza, CE 60714-903, Brazil;Museum National d’Histoire Naturelle, Réserve Zoologique de la Haute Touche, 36290 Obterre, France;

关键词: caprine;    COC;    IVF;    IVP;    IVEP;    oocyte competence;   

DOI  :  10.3390/ani11030873

来源: DOAJ

【 摘 要 】

Reproductive seasonality may have a considerable influence on the efficiency of assisted reproductive technologies in seasonal species. This study evaluated the effect of season on cleavage, blastocyst rates and quality of in vitro produced (IVP) goat embryos. In total, 2348 cumulus–oocyte complexes (COCs) were recovered from slaughterhouse ovaries and subjected to the same IVP system throughout 1.5 years (49 replicates). The odds ratio (OR) among seasons was calculated from values of cleavage and blastocyst rates in each season. Cleavage rate was lower (p < 0.05) in spring (anestrus), in comparison with either autumn (peak of breeding season) or summer, while the winter had intermediate values. Furthermore, lower OR of cleavage was observed in spring. Blastocyst formation rate (from initial number of COCs) was higher (p < 0.05) in autumn (52 ± 2.5%) when compared with the other seasons (combined rates: 40 ± 1.9%). Moreover, its OR was higher (p < 0.05) in autumn compared to all other seasons and impaired in the spring compared to winter (OR: 0.54) and summer (OR: 0.48). Embryo hatchability and blastocyst cell number were similar (p > 0.05) among seasons. In conclusion, the breeding season leads to improved oocyte developmental competence, resulting in higher cleavage and blastocyst yield, whereas embryo quality remained similar throughout the years.

【 授权许可】

Unknown