Photoprotective and antioxidant effects of Rhubarb: inhibitory action on tyrosinase and tyrosine kinase activities and TNF-α, IL-1α and α-MSH production in human melanocytes

BMC Complementary and Alternative Medicine

Research Article

Jéssica PS Silveira¹  Luiz C Di Stasi¹  Leonardo N Seito¹  Gustavo C Dieamant²  Cecília Nogueira²  Samara Eberlin²  Maria CV Pereda² 

[1] Laboratory of Phytomedicines, Department of Pharmacology, Institute of Biosciences, Universidade Estadual Paulista (UNESP), CEP 18618-970, Botucatu, São Paulo, Brazil;Research and Development Department of Chemyunion Chemistry Ltd, Av Independência 1501, CEP 18087-101, Sorocaba, São Paulo, Brazil;

关键词: Rheum rhaponticum;    Skin-lightening;    Photoprotection;    α-MSH;    UV radiation;    Melanocytes;   

DOI  :  10.1186/1472-6882-13-49

 received in 2012-09-28, accepted in 2013-02-26,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundExposure to ultraviolet (UV) radiation causes various forms of acute and chronic skin damage, including immunosuppression, inflammation, premature aging and photodamage. Furthermore, it induces the generation of reactive oxygen species, produces proinflammatory cytokines and melanocyte-stimulating hormone (MSH) and increases tyrosinase activity. The aim of this study was to evaluate the potential photoprotective effects of Rheum rhaponticum L. rhizome extract on human UV-stimulated melanocytes.MethodsThe effects of Rheum rhaponticum rhizome extract on tyrosine kinase activity, and on interleukin-1α (IL-1α), tumour necrosis factor α (TNF-α), and α-MSH production in human epidermal melanocytes were evaluated under UV-stimulated and non-stimulated conditions. Antioxidant activity was evaluated by lipid peroxidation and 1,1-dyphenyl-2-picryl-hydrazyl (DPPH) assays, while anti-tyrosinase activity was evaluated by the mushroom tyrosinase method.ResultsRheum rhaponticum L. rhizome extract showed in vitro antioxidant properties against lipid peroxidation, free radical scavenging and anti-tyrosinase activities, and inhibited the production of IL-1α, TNF-α, α-MSH, and tyrosine kinase activity in melanocytes subjected to UV radiation.ConclusionsThese results support the inclusion of Rheum rhaponticum L. rhizome extract into cosmetic, sunscreen and skin care products for the prevention or reduction of photodamage.

【 授权许可】

Unknown   
© Silveira et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.