Photoprotective and antioxidant effects of Rhubarb: inhibitory action on tyrosinase and tyrosine kinase activities and TNF-α, IL-1α and α-MSH production in human melanocytes

BMC Complementary and Alternative Medicine

Luiz C Di Stasi²  Maria CV Pereda¹  Cecília Nogueira¹  Gustavo C Dieamant¹  Samara Eberlin¹  Leonardo N Seito²  Jéssica PS Silveira² 

[1] Research and Development Department of Chemyunion Chemistry Ltd, Av Independência 1501, Sorocaba, São Paulo CEP 18087-101, Brazil;Laboratory of Phytomedicines, Department of Pharmacology, Institute of Biosciences, Universidade Estadual Paulista (UNESP), Botucatu, São Paulo CEP 18618-970, Brazil

关键词: Melanocytes;    UV radiation;    α-MSH;    Photoprotection;    Skin-lightening;    Rheum rhaponticum;   

Others  :  1230293
DOI  :  10.1186/1472-6882-13-49

 received in 2012-09-28, accepted in 2013-02-26,  发布年份 2013

【 摘 要 】

Background

Exposure to ultraviolet (UV) radiation causes various forms of acute and chronic skin damage, including immunosuppression, inflammation, premature aging and photodamage. Furthermore, it induces the generation of reactive oxygen species, produces proinflammatory cytokines and melanocyte-stimulating hormone (MSH) and increases tyrosinase activity. The aim of this study was to evaluate the potential photoprotective effects of Rheum rhaponticum L. rhizome extract on human UV-stimulated melanocytes.

Methods

The effects of Rheum rhaponticum rhizome extract on tyrosine kinase activity, and on interleukin-1α (IL-1α), tumour necrosis factor α (TNF-α), and α-MSH production in human epidermal melanocytes were evaluated under UV-stimulated and non-stimulated conditions. Antioxidant activity was evaluated by lipid peroxidation and 1,1-dyphenyl-2-picryl-hydrazyl (DPPH) assays, while anti-tyrosinase activity was evaluated by the mushroom tyrosinase method.

Results

Rheum rhaponticum L. rhizome extract showed in vitro antioxidant properties against lipid peroxidation, free radical scavenging and anti-tyrosinase activities, and inhibited the production of IL-1α, TNF-α, α-MSH, and tyrosine kinase activity in melanocytes subjected to UV radiation.

Conclusions

These results support the inclusion of Rheum rhaponticum L. rhizome extract into cosmetic, sunscreen and skin care products for the prevention or reduction of photodamage.

【 授权许可】

   
2013 Silveira et al; licensee BioMed Central Ltd.

【 图 表 】

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【 参考文献 】

• [1]Smith E, Kiss F, Porter RM, Anstey AV: A review of UVA-mediated photosensitivity disorders. Photochem Photobiol Sci 2002, 11:199-206.
• [2]Svobodová A, Vostálová J: Solar radiation induced skin damage: review of protective and preventive options. Int J Radiat Biol 2010, 86:990-1030.
• [3]Giampieri F, Alvarez-Suarez JM, Tulipani S, Gonzáles-Paramàs AM, Santos-Buelga C, Bompadre S, Quiles JL, Mezzetti B, Battino M: Photoprotective potential of strawberry (Fragaria x ananassa) extract against UV-A irradiation damage on human fibroblasts. J Agric Food Chem 2012, 60:2322-2327.
• [4]Niki E: Do antioxidants impair signalling by reactive oxygen species and lipid oxidation products? FEBS Lett 2012, 586:3767-3770.
• [5]Svobodová A, Walterová D, Vostalová J: Ultraviolet light induced alteration to the skin. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 2006, 150:25-38.
• [6]Emir UE, Kurzaz IA: An integrated model for melanocyte-specific gene expression and melanogenesis. Signal Transd 2003, 5–6:209-217.
• [7]De Lucca M, Siegrist W, Bondanza S, Mathor M, Cancedda R, Eberle AN: α melanocyte stimulating hormone (α-MSH) stimulates normal human melanocyte growth by binding to high-affinity receptors. J Cell Sci 1993, 105:1079-1084.
• [8]Halliwell B, Gutteridge JMC: Free radicals in biology and medicine. 4th edition. Osford: Oxford University Press; 2007.
• [9]Chiva-Blanch G, Visioli F: Polyphenols and health: moving beyond antioxidants. J Berry Res 2012, 2:63-71.
• [10]Niki E: Antioxidant capacity: which capacity and how to assess it? J Berry Res 2011, 1:169-176.
• [11]Baurin N, Arnoult E, Scior T, Do QT, Bernard P: Preliminary screening of some tropical plants for anti-tyrosinase activity. J Ethnopharmacol 2002, 2:155-158.
• [12]Dinkova-Kostova AT: Phytochemicals as protectors against ultraviolet radiation: versatility of effects and mechanisms. Planta Med 2008, 74:1548-1559.
• [13]Kim YJ, Uyama H: Tyrosinase inhibitors from natural and synthetic sources: structure, inhibition mechanism and perspective for the future. Cell Mol Life Sci 2005, 62:1707-1723.
• [14]Nichols JA, Katiyar SK: Skin photoprotection by natural polyphenols: anti-inflammatory, antioxidant and DNA repair mechanisms. Arch Dermatol Res 2010, 302:712-783.
• [15]Korac RR, Khambholja KM: Potential of herbs in skin protection from ultraviolet radiation. Pharmacogn Rev 2011, 5:164-173.
• [16]Raal A, Pokk P, Arend A, Aunapuu M, Jõgi J, Ôkva K, Püssa T: Pharmacokinetics of hydroxystilbenes of Rheum rhaponticum L. Eur J Pharm Sci 2007, 32:S24.
• [17]Raal A, Pokk P, Arend A, Aunapuu M, Jõgi J, Ôkva K, Püssa T: Trans-resveratrol alone and hydroxystilbenes of rhubarb (Rhueum rhaponticum L.) root reduce liver damage induced by chronic ethanol administration: a comparative study in mice. Phytother Res 2009, 23:525-532.
• [18]Wober J, Möller F, Richter T, Unger C, Weigt C, Jandausch A, Zierau O, Rettenberger R, Kaszkin-Bettag M, Vollmer G: Activation of estrogen receptor-β by a special extract of Rheum rhaponticum (ERr 731®), its aglycones and structurally related compounds. J Steroid Biochem Mol Biol 2007, 107:191-201.
• [19]Kim YM, Yun J, Lee CK, Lee H, Min KR, Kim Y: Oxyresveratrol and hydroxystilbene compounds. Inhibitory effect on tyrosinase and mechanism of action. J Biol Chem 2002, 277:16340-16344.
• [20]Liu Y, Chan F, Sun H, Yan J, Fan D, Zhao D, An J, Zhou D: Resveratrol protects human keratinocytes HaCaT cells from UVA-induced oxidative stress damage by downregulating Keap1 expression. Eur J Pharmacol 2011, 650:130-137.
• [21]Silveira JEPS, Pereda MCV, Eberlin S, Diemant GC, Di Stasi LC: Effects of Coccoloba uvifera L. on UV-stimulated melanocytes. Photodermatol Photoimmunol Photomed 2008, 24:308-313.
• [22]Peigen X, Liyi H, Liwei W: Ethnopharmacologic study of Chinese rhubarb. J Ethnopharmacol 1984, 10:275-293.
• [23]Witaicenis A, Seito LN, Di Stasi LC: Intestinal anti-inflammatory activity of esculetin and 4-methylesculetin in the trinitrobenzenesulphonic acid model of rat colitis. Chem Biol Interact 2010, 186:211-218.
• [24]Stocks J, Gutterid JM, Sharp RJ, Dormandy TL: Assay using brain homogenate for measuring the antioxidant activity of biological fluids. Clin Sci Mol Med 1974, 47:215-222.
• [25]Blois MS: Antioxidant determinations by the use of a stable free radical. Nature 1958, 26:1199-1200.
• [26]Lee SH, Choi SY, Kim H, Hwang JS, Lee BG, Gao JJ, Kim S: Mulberroside F isolated from the leaves of Morus alba inhibits melanin biosynthesis. Biol Pharm Bull 2002, 25:1045-1048.
• [27]Mosmann T: Rapid colorimetric assay for cellular growth and survival: application to proliferations and cytotoxicity assays. J Immunol Meth 1983, 65:55-63.
• [28]Petit L, Piérard GF: Skin-lightening products revisited. Int J Cosmet Sci 2003, 25:169-181.
• [29]Afaq F, Mukhtar AF: Botanical antioxidants in the prevention of photocarcinogenesis and photoaging. Exp Dermatol 2006, 15:678-684.
• [30]Lin J, Fisher DE: Melanocyte biology and skin pigmentation. Nature 2007, 445:843-850.
• [31]Ito S, Wakamatsu K, Ozeki H: Chemical analysis of melanins and its application to the study of the regulation of melanogenesis. Pigment Cell Res 2000, 13:103-109.
• [32]Chackraborty AK, Funasaka Y, Slominski A, Ermak G, Juang J, Pawelek JM, Ichihashi M: Production and release of pro-piomelanocortin (POMC) derived peptides by human melanocytes and keratinocytes culture: regulation by ultraviolet B. Biochim Biophys Acta 1996, 1313:130-138.
• [33]Tsatmali M, Ancans J, Thody AJ: Melanocyte function and its control by melanocortin peptides. J Histochem Cytochem 2002, 50:125-133.
• [34]Tsatmali M, Ancans J, Yukitake J, Thody AJ: Skin POMC peptides: their actions at the human MC-1 receptor and roles in the tanning response. Pigment Cell Res 2000, 13:125-129.
• [35]Lerner AB, McGuire JS: Effect of alpha- and beta-melanocyte stimulating hormone on the skin colour of man. Nature 1961, 189:176-179.
• [36]Yanase H, Ando H, Horikawa M, Watanabe M, Mori T, Matsuda N: Possible involvement of ERK ½ in UVA-induced melanogenesis in cultured normal human epidermal melanocytes. Pig Cell Res 2001, 10:103-109.
• [37]Reagan-Shaw S, Mukthar H, Ahmad N: Resveratrol imparts photoprotection of normal cells and enhances the efficacy of radiation therapy in cancer cells. Photochem Photobiol 2008, 84:415-421.
• [38]Ohno O, Watanabe T, Nakamura K, Kawagoshi M, Uotsu N, Chiba T, Yamada M, Yamaguchi K, Yamada K, Miyamoto K, Uemura D: Inhibitory effects of bakuchiol, bavachin and isobavachalcone isolated from Piper longum on melanin production in B16 mouse melanoma cells. Biosci Biotechnol Biochem 2010, 74:1504-1506.
• [39]Roh E, Yun CY, Lee JW, Lee C, Hwang BY, Oh ST, Jung SH, Han SB, Kim Y: Hypopigmenting activity of bisabolangelone isolated from Angelica koreana Maxim. in α-melanocyte stimulating hormone-activated B16 or melan-a cells. Planta Med 2011, 77:248-251.
• [40]Yun CY, Kim D, Lee WH, Park YM, Lee SH, Na M, Jahng Y, Hwang BY, Lee MK, Han SB, Kim Y: Torilin from Torilis japonica inhibits melanin production in α-melanocyte stimulating hormone-activated B16 melanoma cells. Planta Med 2009, 75:1505-1508.
• [41]Slominski A, Paus R, Schadendorf D: Melanocytes as “sensory” and regulatory cells in the epidermis. J Theor Biol 1993, 164:103-120.
• [42]Haycock JW, Rowe SJ, Cartlewdge S, Wyatt A, Ghanem G, Morandini R, Rennie IG, MacNeil S: α-melanocyte-stimulating hormone reduces impact of proinflammatory cytokine and peroxide-generated oxidative stress on keratinocyte and melanoma cell lines. J Biol Chem 2000, 275:15629-15636.
• [43]Kim SJ, Son KH, Chang HW, Kang SS, Kim HP: Tyrosinase inhibitory prenylated flavonoids from Sophora flavescens. Biol Pharm Bull 2003, 26:1348-1350.
• [44]Nerya O, Vaya J, Musa R, Izrael S, Ben-Aire R, Tamir S: Glabrene and isoiquiritigenin as tyrosinase inhibitors from licorice roots. J Agric Food Chem 2003, 51:1201-1207.
• [45]Afaq F, Mukhtar H: Effects of solar radiation on cutaneous detoxification pathways. J Photochem Photobiol B Biol 2001, 6:61-69.
• [46]Bickers DR, Athar M: Oxidative stress in the pathogenesis of skin disease. J Invest Dermatol 2006, 126:2565-2575.
• [47]Finklel T, Holbrook NJ: Oxidants, oxidative stress and the biology of ageing. Nature 2000, 408:239-247.
• [48]Adhami VM, Syed AN, Khan N, Afaq F: Phytochemicals for prevention of solar ultraviolet radiation-induced damages. Photochem Photobiol 2008, 84:489-500.
• [49]Öztürk M, Aydogmus-Öztürk F, Duru ME, Topçu G: Antioxidant activity of stem and root extracts of Rhubarb (Rheum ribes): an edible medicinal plant. Food Chem 2007, 103:623-630.