Forskolin as a tool to study the β‐adrenergic receptor‐elicited, labeled protein secretion in rat lacrimal gland

FEBS Letters

Mauduit, Philippe¹  Herman, Geneviève¹  Rossignol, Bernard¹ 

[1] Laboratoire de Biochimie des Transports Cellulaires, Institut de Biochimie, Bât 432, Université de Paris-Sud, 91405 Orsay Cedex, France

关键词: Forskolin;    β-Adrenergic receptor;    Protein secretion;    Rat lacrimal gland;    PGE1;    PGE2;    PGD2 and PGI2;    prostaglandin E1;    E2;    D2;    I2;    cAMP;    3′;    5′-adenosine monophosphate;    ACTH;    adrenocorticotropic hormone;    α-MSH;    α-melanocyte-stimulating hormone;    VIP;    vasoactive intestinal peptide;    TSH;    thyroid-stimulating hormone;   

DOI  :  10.1016/0014-5793(83)80111-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In rat lacrimal glands, Forskolin induces a dose-dependent [³H]protein release. This effect can be potentiated by papaverine. As for the other inducers whose effects on protein secretion are assumed to be cAMP-mediated, Forskolin secretion time course shows a latency. Isoproterenol decreases the Forskolin EC _5- at least 60--times. On the other hand, Forskolin enhances the efficacy of isoproterenol without affecting its potency. As a whole, the data collected show that isoproterenol-induced [³H]protein secretion in rat lacrimal glands involved adenylate cyclase activation by coupling with β-adrenergic receptors.

【 授权许可】

Unknown