Norepinephrine evokes a marked Mg2+ efflux from liver cells

FEBS Letters

Scarpa, A.¹  Romani, A.¹ 

[1] Department of Physiology and Biophysics, Case Western Reserve University, School of Medicine, Cleveland, OH 44106, USA

关键词: Mg2+;    Hepatocyte;    β-Adrenergic receptor;    cAMP;    Rat liver;    Hepes;    4-(2-hydroxymethyl)-1- piperazine ethane sulphonic acid;    8-Cl-cAMP;    8-(4-chlorophenylthio)-adenosine 3':5'-cyclic monophosphate;    8-Br-cAMP;    8-bromoadenosine 3':5' cyclic monophosphate;    dBucAMP;    N 6;    2'-O-dibutyryladenosine 3':5' -cyclic monophosphate;   

DOI  :  10.1016/0014-5793(90)81113-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The addition of norepinephrine to perfused rat livers and to collagenase isolated hepatocytes induced a marked and dose-dependent magnesium efflux. The addition of β-adrenergic receptor antagonists, but not α-antagonists, completely blocked the Mg²⁺ efflux. The Mg²⁺ efflux could also be induced by forskolin and by permeable cAMP analogues. By contrast, the addition of carbachol or vasopressin induced a Mg²⁺ influx into isolated hepatocytes. These results indicate that a significant Mg²⁺ efflux from liver cells can be induced through the β-adrenergic receptors and that it is mediated through the cytosolic cAMP levels.

【 授权许可】

Unknown