Study on the antiviral activity of San Huang Yi Gan Capsule against hepatitis B virus with seropharmacological method

BMC Complementary and Alternative Medicine

Research Article

Haibo Xu¹  Cheng Peng¹  Qinghe Wu²  Lijuan Zhou³ 

[1] Department of Pharmacology, College of Pharmacy, Chengdu University of Traditional Chinese Medicine, 1166 Liutai Avenue, 611137, Wenjiang, Chengdu, Sichuan, P.R. China;Department of Pharmacology, College of Pharmacy, Guangzhou University of Chinese Medicine, Guangzhou, China;Institute for Chinese Medicine Research, Sichuan Academy of Chinese Medicine Sciences, Chengdu, China;

关键词: Entecavir;    Hepatitis B virus;    San Huang Yi Gan capsule;    Seropharmacology;   

DOI  :  10.1186/1472-6882-13-239

 received in 2012-07-10, accepted in 2013-09-24,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundSeropharmacology arising recently is a novel method of in vitro pharmacological study on Chinese herb using drug-containing animal serum. As seropharmacology possesses the advantages of experiments in vitro and in vivo, it is increasingly applied in pharmacological research on Chinese medicine. However, some issues of seropharmacology remain controversial and need to be clearly defined. San Huang Yi Gan Capsule (SHYGC) is a Chinese herbal formula with antiviral property against hepatitis B virus (HBV), but little is known about the mechanism underlying its anti-HBV activity. The aim of the present study was to elucidate the action mechanism of SHYGC using seropharmacological method and systematically address the methodology of preparing drug-containing serum.MethodsNew Zealand rabbits were orally administrated SHYGC with various regimens, followed by preparation of SHYGC-containing rabbit sera with a variety of methods. After HBV-producing HepG2 2.2.15 cells were treated with SHYGC-containing sera or entecavir for 9 days, the levels of hepatitis B surface antigen (HBsAg) and HBV DNA and the activity of DNA Polymerase were determined in HepG2 2.2.15 cells-conditioned media.ResultsAn optimally standardized method of preparing drug-containing serum was raised for seropharmacology, with which SHYGC was demonstrated to suppress HBsAg expression, HBV DNA replication and DNA Polymerase activity in a dose-dependent fashion.ConclusionsThis seropharmacological study shows SHYGC is a potentially powerful anti-HBV agent. Additionally, seropharmacology is a promising pharmacological method with a broad range of advantages, and it can be widely used in biomedical research, if combined with pharmacokinetics.

【 授权许可】

CC BY   
© Xu et al.; licensee BioMed Central Ltd. 2013