Immunogenicity of antigens from the TbD1 region present in M. africanum and missing from "modern" M. tuberculosis: a cross- sectional study

BMC Infectious Diseases

Research Article

Jacob K Otu¹  Richard A Adegbola¹  Martin Antonio¹  Abdulrahman Hammond²  Martin O Ota³  Bouke C de Jong³ 

[1] Bacterial Diseases Programme, MRC Laboratories, POB 273, Banjul, Gambia;Bacterial Diseases Programme, MRC Laboratories, POB 273, Banjul, Gambia;FMPOS, University of Bamako, Point G, Bamako, Mali;Bacterial Diseases Programme, MRC Laboratories, POB 273, Banjul, Gambia;New York University, 550 First Avenue, Smilow 901, 10016, New York, NY, USA;

关键词: Tuberculosis;    Latent Infection;    ELISPOT Assay;    Dual Infection;    Interferon Gamma Release Assay;   

DOI  :  10.1186/1471-2334-10-11

 received in 2009-06-02, accepted in 2010-01-19,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundCurrently available tools cannot be used to distinguish between sub-species of the M. tuberculosis complex causing latent tuberculosis (TB) infection. M. africanum causes up to half of TB in West- Africa and its relatively lower progression to disease suggests the presence of a large reservoir of latent infection relative to M. tuberculosis.MethodsWe assessed the immunogenicity of the TbD1 region, present in M. africanum and absent from "modern" M. tuberculosis, in an ELISPOT assay using cells from confirmed M. africanum or M. tuberculosis infected TB patients without HIV infection in the Gambia.ResultsAntigens from the TbD1 region induced IFNγ responses in only 35% patients and did not discriminate between patients infected with M. africanum vs. M. tuberculosis, while PPD induced universally high responses.ConclusionsFurther studies will need to assess other antigens unique to M. africanum that may induce discriminatory immune responses.

【 授权许可】

CC BY   
© de Jong et al; licensee BioMed Central Ltd. 2010