Antioxidant and antimicrobial activities of ethyl acetate extract, fractions and compounds from stem bark of Albizia adianthifolia (Mimosoideae)

BMC Complementary and Alternative Medicine

Research Article

Jean de Dieu Tamokou¹  Jules Roger Kuiate¹  Paul Keilah Lunga²  Mathieu Tene³  Pierre Tane³  Deke James Simo Mpetga³ 

[1] Department of Biochemistry, Laboratory of Microbiology and Antimicrobial Substances, Faculty of Science, University of Dschang, PO Box 67, Dschang, Cameroon;Department of Biochemistry, Laboratory of Microbiology and Antimicrobial Substances, Faculty of Science, University of Dschang, PO Box 67, Dschang, Cameroon;Department of Biochemistry, Laboratory of Phytobiochemistry and Medicinal Plant Study, Faculty of Science, University of Yaounde I, PO Box 812, Yaounde, Cameroon;Department of Chemistry, Laboratory of Natural Product Chemistry, Faculty of Science, University of Dschang, PO Box 67, Dschang, Cameroon;

关键词: Albizia adianthifolia;    Antioxidant;    Antimicrobial;    Phenols;    Fractionation;    Aurantiamide acetate;   

DOI  :  10.1186/1472-6882-12-99

 received in 2012-04-05, accepted in 2012-06-30,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundAlbizia adianthifolia is used traditionally in Cameroon to treat several ailments, including infectious and associated diseases. This work was therefore designed to investigate the antioxidant and antimicrobial activities of ethyl acetate extract, fractions and compounds isolated from the stem bark of this plant.MethodsThe plant extract was prepared by maceration in ethyl acetate. Its fractionation was done by column chromatography and the structures of isolated compounds were elucidated using spectroscopic data in conjunction with literature data. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) and trolox equivalent antioxidant capacity (TEAC) assays were used to detect the antioxidant activity. Broth micro-dilution method was used for antimicrobial test. Total phenol content was determined spectrophotometrically in the extracts by using Folin–Ciocalteu method.ResultsThe fractionation of the extract afforded two known compounds: lupeol (1) and aurantiamide acetate (2) together with two mixtures of fatty acids: oleic acid and n-hexadecanoic acid (B1); n-hexadecanoic acid, octadecanoic acid and docosanoic acid (B2). Aurantiamide acetate was the most active compound. The total phenol concentration expressed as gallic acid equivalents (GAE) was found to vary from 1.50 to 13.49 μg/ml in the extracts. The antioxidant activities were well correlated with the total phenol content (R2 = 0.946 for the TEAC method and R2 = 0.980 for the DPPH free-radical scavenging assay).ConclusionsOur results clearly reveal that the ethyl acetate extract from the stem bark of A. adianthifolia possesses antioxidant and antimicrobial principles. The antioxidant activity of this extract as well as that of compound 2 are being reported herein for the first time. These results provide promising baseline information for the potential use of this plant as well as compound 2 in the treatment of oxidative damage and infections associated with the studied microorganisms.

【 授权许可】

CC BY   
© Tamokou et al.; licensee BioMed Central Ltd. 2012