Antioxidant and antimicrobial activities of ethyl acetate extract, fractions and compounds from stem bark of Albizia adianthifolia (Mimosoideae)

BMC Complementary and Alternative Medicine

Jules Roger Kuiate³  Pierre Tane²  Mathieu Tene²  Paul Keilah Lunga¹  Deke James Simo Mpetga²  Jean de Dieu Tamokou³ 

[1]Department of Biochemistry, Laboratory of Phytobiochemistry and Medicinal Plant Study, Faculty of Science, University of Yaounde I, PO Box 812, Yaounde, Cameroon

[2]Department of Chemistry, Laboratory of Natural Product Chemistry, Faculty of Science, University of Dschang, PO Box 67, Dschang, Cameroon

[3]Department of Biochemistry, Laboratory of Microbiology and Antimicrobial Substances, Faculty of Science, University of Dschang, PO Box 67, Dschang, Cameroon

关键词: Aurantiamide acetate;    Fractionation;    Phenols;    Antimicrobial;    Antioxidant;    Albizia adianthifolia;   

Others  :  1232116
DOI  :  10.1186/1472-6882-12-99

 received in 2012-04-05, accepted in 2012-06-30,  发布年份 2012

【 摘 要 】

Background

Albizia adianthifolia is used traditionally in Cameroon to treat several ailments, including infectious and associated diseases. This work was therefore designed to investigate the antioxidant and antimicrobial activities of ethyl acetate extract, fractions and compounds isolated from the stem bark of this plant.

Methods

The plant extract was prepared by maceration in ethyl acetate. Its fractionation was done by column chromatography and the structures of isolated compounds were elucidated using spectroscopic data in conjunction with literature data. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) and trolox equivalent antioxidant capacity (TEAC) assays were used to detect the antioxidant activity. Broth micro-dilution method was used for antimicrobial test. Total phenol content was determined spectrophotometrically in the extracts by using Folin–Ciocalteu method.

Results

The fractionation of the extract afforded two known compounds: lupeol (1) and aurantiamide acetate (2) together with two mixtures of fatty acids: oleic acid and n-hexadecanoic acid (B₁); n-hexadecanoic acid, octadecanoic acid and docosanoic acid (B₂). Aurantiamide acetate was the most active compound. The total phenol concentration expressed as gallic acid equivalents (GAE) was found to vary from 1.50 to 13.49 μg/ml in the extracts. The antioxidant activities were well correlated with the total phenol content (R² = 0.946 for the TEAC method and R² = 0.980 for the DPPH free-radical scavenging assay).

Conclusions

Our results clearly reveal that the ethyl acetate extract from the stem bark of A. adianthifolia possesses antioxidant and antimicrobial principles. The antioxidant activity of this extract as well as that of compound 2 are being reported herein for the first time. These results provide promising baseline information for the potential use of this plant as well as compound 2 in the treatment of oxidative damage and infections associated with the studied microorganisms.

【 授权许可】

   
2012 Tamokou et al.; licensee BioMed Central Ltd.

【 图 表 】

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【 参考文献 】

• [1]Zabala NQ: Albizia and Paraserianthes species. Proceedings of an international workshop held in Bislig, Suriqao; del Sur, Philippines; 1997:13-19.
• [2]Watt JM, Breyer-Brandwyk MG: The medicinal and poisonous plants of Southern and Easthern Africa. 2nd edition. Livingstone, London; 1962.
• [3]Van-Wyk BE, Gerick N: People’s plants: a guide to useful plants of Southern Africa. Briza publications, Pretoria; 2000.
• [4]Haddad M, Miyamoto T, Laurens V, Lacaille-Dubois MA: Two new biologically active triterpenoidal saponins acylated with salicylic acid from Albizia adianthifolia. J Nat Prod 2003, 66:372-377.
• [5]Candy HA, Brookes KB, Bull JR, Mc Garry EJ, Mc Garry JM: Flavonoids of Albizia adianthifolia. Phytochemistry 1978, 17:1681-1682.
• [6]Eldeen IMS, Elgarashi EE, Van-Staden J: Antibacterial, anti-inflammatory, anti-cholinesterase and mutagenic effects of extracts obtained from some trees used in South African traditional medicine. J Ethnopharmacol 2005, 102:457-464.
• [7]Kishore N, Chansouria JPN, Dubey NK: Antidermatophytic action of the essential oil of Chenopodium ambrosioides and an ointment prepared from it. Phytother Res 1996, 10:453-455.
• [8]Soberon JR, Sgariglia MA, Sampietro DA, Quiroga EN, Vattuone MA: Antibacterial activity of plant extracts from Northwestern Argentina. J Appl Microbiol 2007, 102:1450-1461.
• [9]Holliinworth H: The management of infected wounds. Professional Nurse 1997, 12:8-11.
• [10]Sholichin M, Yamasaki K, Kasai R, Tanaka O: 13C Nuclear magnetic resonance of lupine-type triterpenes, lupeol, betulin and betulinic acid. Chem Pharm Bull 1980, 28:1006-1008.
• [11]Banerji A, Ray R: Aurantiamide a new class of modified dipeptides from Piper aurantiacum. Phytochemistry 1981, 20:2217-2220.
• [12]International Organization for Standardization (ISO) 14502–1: Determination of substances characteristic of green and black tea. Part 1: Content of total polyphenols in tea. Colorimetric method using Folin-Ciocalteu reagent; 2005.
• [13]Tamokou JD, Tala FM, Wabo KH, Kuiate JR, Tane P: Antimicrobial activities of methanol extract and compounds from stem bark of Vismia rubescens. J Ethnopharmacol 2009, 124:571-575.
• [14]Nyaa TBL, Tapondjou AL, Barboni L, Tamokou JD, Kuiate JR, Tane P, Park HJ: NMR assignment and Antimicrobial/antioxidant activities of 1β-hydroxyeuscaphic acid from the seeds of Butyrospermum parkii. Nat Prod Sci 2009, 15:76-82.
• [15]Rice-Evans C, Miller NJ: Total antioxidant status in plasma and body fluids. Methods Enzymol 1994, 234:279-293.
• [16]Singleton VL, Orthofer R, Lamuela-Ravento’s RM: Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Method Enzymol 1999, 299:152-178.
• [17]Carbonnelle B, Denis F, Marmonier A, Pinon G, Vague R: Bactériologie médicale: Techniques usuelles. SIMEP, Paris; 1987.
• [18]Tene M, Tane P, Tamokou JD, Kuiate JR, Connolly JD: Degraded diterpenoids from the stem bark of Neoboutonia mannii. Phytochemistry Lettters 2008, 1:120-124.
• [19]Kabara JJ: Toxicological, bacteriological and fungicidal properties of fatty acids and some derivatives. J Am Oil Chem Soc 1979, 56:760A-767A.
• [20]Nazif NM: Phytoconstituents of Zizyphus spina-christi L. fruits and their antimicrobial activity. Food Chem 2002, 76:77-81.
• [21]Ragasa CY, Penalosa BA, Rideout JA: A bioactive dipeptide derivative from Malachra fasciata. Philipp J Sci 1998, 127:267-276.
• [22]Singh B, Singh S: Antimicrobial activity of terpenoids from Trichodesma amplexicaule Roth. Phytother Res 2003, 17:814-816.
• [23]Jung MJ, Chung HY, Kang SS, Choi JH, Bae KS, Choi JS: Antioxidant activity from the stem bark of Albizia julibrissin. Arch Pharm Res 2003, 26:458-462.
• [24]Roginsky V, Lissi EA: Review of methods to determine chainbreaking antioxidant activity in food. Food Chem 2005, 92:235-254.
• [25]Chen H, Ho CT: Comparative Study on Total Polyphenol Content and Total Antioxidant Activity of Tea (Camellia sinensis). In Antioxidant Measurement and Applications; ACS Symposium Series 956. American Chemical Society, Washington, DC; 2007:195-214.
• [26]Hall CA, Cuppett SL: Structure activities of natural antioxidants. In Antioxidant Methodology in Vitro Concepts. Edited by Hudson BJL. Elsevier Applied Science, London; 1997:1-18.
• [27]Pietta P, Sionetti P, Mauri P: Antioxidant activity of selected medicinal plants. J Agric Food Chem 1998, 46:4487-4490.
• [28]Bacq-Calberg CM, Coyotte J, Hoet P, Nguyem-Disteeche M: Microbiology. De Boeck and Larcier, Bruxelle; 1999.
• [29]Savafi L, Duran N, Savafi N, Onlem Y, Ocak S: The prevalence and resistance patterns of Pseudomanas aeruginosa in intensive care units in a university Hospital. Turk J Med Sci 2005, 35:317-322.
• [30]Tamokou JD, Kuiate JR, Tene M, Nwemeguela KTJ, Tane P: The Antimicrobial activities of extract and compounds isolated from Brillantaisia lamium. Iran J Med Sci 2011, 36:24-31.
• [31]Mohammad AH, Shigefumi M, Kotaro M, Hiroshi K, Eisuke S, Kazunori T, Takayoshi T, Shigera K: In vitro and in vivo activities of SCH56592against Cryptococcus neoformans. J Antimicrob Chemother 1999, 44:827-829.