Microarray expression profiling in the denervated hippocampus identifies long noncoding RNAs functionally involved in neurogenesis

BMC Molecular Biology

Research Article

Bingying Deng¹  Xiang Cheng¹  Meiling Tian¹  Jianbing Qin¹  Haoming Li¹  Guohua Jin² 

[1] Department of Anatomy and Neurobiology, The Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, 226001, Nantong, Jiangsu, People’s Republic of China;Department of Anatomy and Neurobiology, The Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, 226001, Nantong, Jiangsu, People’s Republic of China;Medical School of Nantong University, Room 325, Building 3, No. 19 Qixiu Road, Congchuan District, 226001, Nantong, China;

关键词: Hippocampus;    Long noncoding RNA;    Microarray;    Neural stem cells;    Neurogenesis;   

DOI  :  10.1186/s12867-017-0091-2

 received in 2016-06-30, accepted in 2017-05-05,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThe denervated hippocampus provides a proper microenvironment for the survival and neuronal differentiation of neural progenitors. While thousands of lncRNAs were identified, only a few lncRNAs that regulate neurogenesis in the hippocampus are reported. The present study aimed to perform microarray expression profiling to identify long noncoding RNAs (lncRNAs) that might participate in the hippocampal neurogenesis, and investigate the potential roles of identified lncRNAs in the hippocampal neurogenesis.ResultsIn this study, the profiling suggested that 74 activated and 29 repressed (|log fold-change|>1.5) lncRNAs were differentially expressed between the denervated and the normal hippocampi. Furthermore, differentially expressed lncRNAs associated with neurogenesis were found. According to the tissue-specific expression profiles, and a novel lncRNA (lncRNA2393) was identified as a neural regulator in the hippocampus in this study. The expression of lncRNA2393 was activated in the denervated hippocampus. FISH showed lncRNA2393 specially existed in the subgranular zone of the dentate gyrus in the hippocampus and in the cytoplasm of neural stem cells (NSCs). The knockdown of lncRNA2393 depletes the EdU-positive NSCs. Besides, the increased expression of lncRNA2393 was found to be triggered by the change in the microenvironment.ConclusionWe concluded that expression changes of lncRNAs exists in the microenvironment of denervated hippocampus, of which promotes hippocampal neurogenesis. The identified lncRNA lncRNA2393 expressed in neural stem cells, located in the subgranular zone of the dentate gyrus, which can promote NSCs proliferation in vitro. Therefore, the question is exactly which part of the denervated hippocampus induced the expression of lncRNA2393. Further studies should aim to explore the exact molecular mechanism behind the expression of lncRNA2393 in the hippocampus, to lay the foundation for the clinical application of NSCs in treating diseases of the central nervous system.

【 授权许可】

CC BY   
© The Author(s) 2017