Unraveling the early molecular and physiological mechanisms involved in response to phenanthrene exposure

BMC Genomics

Research Article

Guillem Rigaill¹  Ludivine Taconnat¹  Richard Berthomé²  Evangelos Barbas³  Olivier Catrice⁴  Anne-Sophie Dumas⁵  Abdelhak El Amrani⁵  Delphine Bernard⁶  David Macherel⁷  Abdelilah Benamar⁷ 

[1] Institute of Plant Sciences Paris Saclay (IPS2), UMR 9213/UMR1403, Université Paris Sud, CNRS, INRA, Université d’Evry, Université Paris Diderot, Sorbonne Paris Cité, Bâtiment 630, 91405, Orsay, France;Institute of Plant Sciences Paris Saclay (IPS2), UMR 9213/UMR1403, Université Paris Sud, CNRS, INRA, Université d’Evry, Université Paris Diderot, Sorbonne Paris Cité, Bâtiment 630, 91405, Orsay, France;Laboratoire des Interactions Plantes Micro-organismes (LIPM), UMR INRA 441/CNRS 2594, CS 52627, 31326, Castanet Tolosan cedex, France;Institute of Plant Sciences Paris Saclay (IPS2), UMR 9213/UMR1403, Université Paris Sud, CNRS, INRA, Université d’Evry, Université Paris Diderot, Sorbonne Paris Cité, Bâtiment 630, 91405, Orsay, France;Present Address: Laboratory of Forest Genetics and Tree Breeding, AUTH, University Campus, 54124, Thessaloniki, Greece;Laboratoire des Interactions Plantes Micro-organismes (LIPM), UMR INRA 441/CNRS 2594, CS 52627, 31326, Castanet Tolosan cedex, France;Université de Rennes 1, CNRS/OSUR/UMR 6553, Ecosystèmes-Biodiversité-Evolution, campus de Beaulieu, Bâtiment 14A, 35042, Rennes cedex, France;Université de Rennes 1, CNRS/OSUR/UMR 6553, Ecosystèmes-Biodiversité-Evolution, campus de Beaulieu, Bâtiment 14A, 35042, Rennes cedex, France;Present Address: Laboratoire de Génétique Moléculaire et de Génétique Epidémiologique, INSERM U1078, 46, rue Felix Le Dantec, CS 51819, 29218, Brest Cedex 2, France;Université d’Angers, UMR 1345, Institut de Recherche en Horticulture et Semences (IRHS), Bat ARES, 16 Boulevard Lavoisier, 49045, Angers cedex, France;

关键词: Abiotic stress;    Phenanthrene;    Phytoremediation;    Arabidopsis;    Energy availability;    Transcriptome;    Xenome;    Metabolites;   

DOI  :  10.1186/s12864-016-3133-0

 received in 2016-05-05, accepted in 2016-09-27,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundHigher plants have to cope with increasing concentrations of pollutants of both natural and anthropogenic origin. Given their capacity to concentrate and metabolize various compounds including pollutants, plants can be used to treat environmental problems - a process called phytoremediation. However, the molecular mechanisms underlying the stabilization, the extraction, the accumulation and partial or complete degradation of pollutants by plants remain poorly understood.ResultsHere, we determined the molecular events involved in the early plant response to phenanthrene, used as a model of polycyclic aromatic hydrocarbons. A transcriptomic and a metabolic analysis strongly suggest that energy availability is the crucial limiting factor leading to high and rapid transcriptional reprogramming that can ultimately lead to death. We show that the accumulation of phenanthrene in leaves inhibits electron transfer and photosynthesis within a few minutes, probably disrupting energy transformation.ConclusionThis kinetic analysis improved the resolution of the transcriptome in the initial plant response to phenanthrene, identifying genes that are involved in primary processes set up to sense and detoxify this pollutant but also in molecular mechanisms used by the plant to cope with such harmful stress. The identification of first events involved in plant response to phenanthrene is a key step in the selection of candidates for further functional characterization, with the prospect of engineering efficient ecological detoxification systems for polycyclic aromatic hydrocarbons.

【 授权许可】

CC BY   
© The Author(s). 2016