Effect of bioaugmentation to enhance phytoremediation for removal of phenanthrene and pyrene from soil with Sorghum and Onobrychis sativa

Journal of Environmental Health Science Engineering

Ali Esrafili³  Nemat Jaafarzadeh²  Simin Nasseri¹  Ahmad Jonidi Jafari³  Roshanak Rezaei Kalantary³  Mohammad Mehdi Baneshi³ 

[1] Center for Water Quality Research, Institute of Environmental Research, Tehran University of Medical Sciences, Tehran, Iran;Environmental Technology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran;Department of Environmental Health Engineering, School of Public Health, Iran University of Medical Sciences, Tehran, Iran

关键词: Sorghum;    Soil;    Pyrene;    Phytoremediation;    Phenanthrene;    Onobrychis sativa;    Bioaugmentation;   

Others  :  810604
DOI  :  10.1186/2052-336X-12-24

 received in 2013-07-08, accepted in 2013-10-22,  发布年份 2014

【 摘 要 】

The use of plants to remove Poly-aromatic-hydrocarbons (PAHs) from soil (phytoremediation) is emerging as a cost-effective method. Phytoremediation of contaminated soils can be promoted by the use of adding microorganisms with the potential of pollution biodegradation (bioaugmentation). In the present work, the effect of bacterial consortium was studied on the capability of Sorghum and Onobrychis sativa for the phytoremediation of soils contaminated with phenanthrene and pyrene. 1.5 kg of the contaminated soil in the ratio of 100 and 300 mg phenanthrene and/or pyrene per kg of dry soil was then transferred into each pot (nine modes). The removal efficiency of natural, phytoremediation and bioaugmentation, separately and combined, were evaluated. The samples were kept under field conditions, and the remaining concentrations of pyrene and phenanthrene were determined after 120 days. The rhizosphere as well as the microbial population of the soil was also determined. Results indicated that both plants were able to significantly remove pyrene and phenanthrene from the contaminated soil samples. Phytoremediation alone had the removal efficiency of about 63% and 74.5% for pyrene and phenanthrene respectively. In the combined mode, the removal efficiency dramatically increased, leading to pyrene and phenanthrene removal efficiencies of 74.1% and 85.02% for Onobrychis sativa and 73.84% and 85.2% for sorghum, respectively. According to the results from the present work, it can be concluded that Onobrychis sativa and sorghum are both efficient in removing pyrene and phenanthrene from contamination and bioaugmentation can significantly enhance the phytoremediation of soils contaminated with pyrene and phenanthrene by 22% and 16% respectively.

【 授权许可】

   
2014 Baneshi et al.; licensee BioMed Central Ltd.

【 图 表 】

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【 参考文献 】

• [1]Sheng-wang PAN, Shi-qiang WEI, Xin Y, Sheng-xian CAO: The removal and remediation of phenanthrene and pyrene in soil by mixed cropping of alfalfa and rape. Agri Sci China 2008, 7:1355-1364.
• [2]Zhang J, Yin R, Lin X, Liu W, Chen R: Intractive effect of biosurfactant and microorganism to enhanced phytoremediation for removal of aged polycyclic aromatic hydrocarbons from contaminated soils. J Health Sci 2010, 56:257-266.
• [3]Nasseri S, Kalantary R, Nourieh N, Naddafi K, Mahvi A, Baradaran N: Influence of bioaugmentation in biodegradation of PAHs-contaminated soil in bio-slurry phase reactor. J Environ Health Sci Eng 2010, 7:199-208.
• [4]Dehghanifard E, Jonidi Jafari A, Rezaei Kalantary R, Mahvi AH, Faramarzi MA, Esrafili A: Biodegradation of 2, 4-dinitrophenol with laccase immobilized on nano-porous silica beads. J Environ Health Sci Eng 2013, 10:25. BioMed Central Full Text
• [5]Mahvi AH, Maleki A, Alimohamadi M, Ghasri A: Photo-oxidation of phenol in aqueous solution: toxicity of intermediates. Korean J Chem Eng 2007, 24:79-82.
• [6]Mohseni Bandpi A, Rezaei Kalantary R, Ameli A, Esrafili A, Zinatizadeh AA, Jonidi Jafari A: Application of response surface methodology for optimization of fenton process for phenanthrene removal from soil. Environ Eng Manag J 2013. In Press
• [7]Yu XZ, Wu SC, Wu FY, Wong MH: Enhanced dissipation of PAHs from using mycorrhizal ryegrass and PAH-degrading bacteria. J Hazard Mater 2010. In Press
• [8]Moreira IT, Oliveira O, Triguis JA, Queiroz AF, Ferreira SL, Martins C, Silva A, Falcão BA: Phytoremediation in mangrove sediments impacted by persistent total petroleum hydrocarbons (TPH’s) using Avicennia schaueriana. Mar Pollut Bull 2013, 67:130-136.
• [9]Patil AV, Jadhav JP: Evaluation of phytoremediation potential of tagetes patula L. for the degradation of textile dye reactive blue 160 and assessment of the toxicity of degraded metabolites by cytogenotoxicity. Chemosphere 2013, 92:225-232.
• [10]Zhang D, Zhu L, Li F: Influences and mechanisms of surfactants on pyrene biodegradation based on interactions of surfactant with a klebsiella oxytoca strain. Bioresour Technol 2013, 142:454-461.
• [11]Euliss K, C-h H, Schwab AP, Rock S, Banks MK: Greenhouse and field assessment of phytoremediation for petroleum contaminants in a riparian zone. Bioresource Technol 2008, 99:1961-1971.
• [12]Lee S-H, Lee W-S, Lee C-H, Kim J-G: Degradation of phenanthrene and pyrene in rhizosphere of grasses and legumes. J Hazard Mater 2008, 153:892-898.
• [13]Moghimi J: Introduce some important Rangelands species for improved rangelands development in Iran. Tehran: Arvan Pub; 2005.
• [14]Nourmohammadi G, Siadat A, Almasi M: Agronomy: Cereal crops. Ahvaz: Shahid Chamran University; 2005.
• [15]Heidari Sharifabad H, Dorri MA: Forage legumes. In Book Forage legumes. Tehran: Research Institute of forests and rangeland; 2003.
• [16]Silva IS, Santos EC, Menezes CR, Faria AF, Franciscon E, Grossman M, Durrant LR: Bioremediation of a polyaromatic hydrocarbon contaminated soil by native soil microbiota and bioaugmentation with isolated microbial consortia. Bioresour Technol 2009, 100:4669-4675.
• [17]Yu KSH, Wong AHY, Yau KWY, Wong YS, Tam NFY: Natural attenuation, biostimulation and bioaugmentation on biodegradation of polycyclic aromatic hydrocarbons (PAHs) in mangrove sediments. Mar Pollut Bull 2005, 51:1071-1077.
• [18]Rezaei Kalantary R, Badkoubi A, Mohseni-Bandpi A, Esrafili A, Jorfi S, Dehghanifard E, Baneshi MM: Modification of PAHs biodegradation with humic compounds. Soil Sediment Con Int J 2013, 22:185-198.
• [19]Peng S, Zhou Q, Cai Z, Zhang Z: Phytoremediation of petroleum contaminated soils by Mirabilis Jalapa L. in a greenhouse plot experiment. J Hazard Mater 2009, 168:1490-1496.
• [20]Eweis JB, Ergas SJ, Chang DPY, Schroeder ED: Bioremediation principles. Europe: McGraw-Hill Book Company; 1998.
• [21]Cheema SA, Khan MI, Tang X, Zhang C, Shen C, Malik Z, Ali S, Yang J, Shen K, Chen X, Chen Y: Enhancement of phenanthrene and pyrene degradation in rhizosphere of tall fescue (Festuca arundinacea). J Hazard Mater 2009, 166:1226-1231.
• [22]Khan S, Afzal M, Iqbal S, Mirza MS, Khan QM: Inoculum pretreatment affects bacterial survival, activity and catabolic gene expression during phytoremediation of diesel contaminated soil. Chemosphere 2013, 91:663-668.
• [23]Tahhan RA, Ammari TG, Goussous SJ, Al-Shdaifat HI: Enhancing the biodegradation of total petroleum hydrocarbons in oily sludge by a modified bioaugmentation strategy. Int Biodeter Biodegr 2011, 65:130-134.
• [24]Teng Y, Luo Y, Sun M, Liu Z, Li Z, Christie P: Effect of bioaugmentation by Paracoccus sp. strain HPD-2 on the soil microbial community and removal of polycyclic aromatic hydrocarbons from an aged contaminated soil. Bioresour Technol 2010, 101:3437-3443.
• [25]Venkata Mohan S, Prasanna D, Purushotham Reddy B, Sarma PN: Ex situ bioremediation of pyrene contaminated soil in bio-slurry phase reactor operated in periodic discontinuous batch mode: Influence of bioaugmentation. Int Biodeter Biodegr 2008, 62:162-169.
• [26]Jha P, Jobby R, Kudale S, Modi N, Dhaneshwar A, Desai N: Biodegradation of phenol using hairy roots of Helianthus annuus L. Int Biodeter Biodegr 2013, 77:106-113.
• [27]Mohsenzadeh F, Nasseri S, Mesdaghinia A, Nabizadeh R, Zafari D, Khodakaramian G, Chehregani A: Phytoremediation of petroleum-polluted soils: Application of Polygonum aviculare and its root-associated (penetrated) fungal strains for bioremediation of petroleum-polluted soils. Ecotox Environ Safe 2010, 73:613-619.
• [28]Vandenhove H: Phytoremediation options for radioactively contaminated sites evaluated. Ann Nucl Energy 2013. In Press
• [29]Liste H-H, Alexander M: Plant-promoted pyrene degradation in soil. Chemosphere 2000, 40:7-10.
• [30]Lu S, Teng Y, Wang J, Sun Z: Enhancement of pyrene removed from contaminated soils by bidens maximowicziana. Chemosphere 2010, 81:645-650.
• [31]Borras E, Caminal G, Sarra M, Novotny C: Effect of soil bacteria on the ability of polycyclic aromatic hydrocarbons (PAHs) removal by trametes versicolor and irpex lacteus from contaminated soil. Soil Biol Biochem 2010, 42:2087-2093.
• [32]Xu Y, Lu M: Bioremediation of crude oil-contaminated soil: comparison of different biostimulation and bioaugmentation treatments. J Hazard Mater 2010, 183:395-401.
• [33]Siciliano S, Germida J: Mechanisms of phytoremediation: biochemical and ecological interactions between plants and bacteria. Environ Rev 1998, 6:65-79.
• [34]Kaimi E, Mukaidani T, Miyoshi S, Tamaki M: Ryegrass enhancement of biodegradation in diesel-contaminated soil. Environ Exp Bot 2006, 55:110-119.
• [35]Hamdi H, Benzarti S, Manusadžianas L, Aoyama I, Jedidi N: Bioaugmentation and biostimulation effects on PAH dissipation and soil ecotoxicity under controlled conditions. Soil Biol Biochem 2007, 39:1926-1935.
• [36]Janbandhu A, Fulekar MH: Biodegradation of phenanthrene using adapted microbial consortium isolated from petrochemical contaminated environment. J Hazard Mater 2011, 187:333-340.
• [37]Gao Y, Yu XZ, Wu SC, Cheung KC, Tam NFY, Qian PY, Wong MH: Interactions of rice (Oryza sativa L.) and PAH-degrading bacteria (Acinetobacter sp.) on enhanced dissipation of spiked phenanthrene and pyrene in waterlogged soil. Sci Total Environ 2006, 372:1-11.