Fourier Transform Mid-Infrared (FTIR) Spectroscopy to Identify Tannin Compounds in The Panicle of Sorghum Mutant Lines

9th Annual Basic Science International Conference 2019

自然科学（总论）

Wahyono, Teguh^1^2 ; Astuti, Dewi Apri^3 ; Komang Gede Wiryawan, I.^3 ; Sugoro, Irawan^2 ; Jayanegara, Anuraga^3

Graduate School of Nutrition and Feed Science, Faculty of Animal Science, Bogor Agricultural University, Bogor

16680, Indonesia^1

Agricultural Division, Center for Isotopes and Radiation Application, National Nuclear Energy Agency, Jakarta

12440, Indonesia^2

Departement of Nutrition and Feed Technology, Faculty of Animal Science, Bogor Agricultural University, Indonesia^3

关键词: Condensed tannins;    FTIR;    Harvesting age;    Maturity stages;    Mutant lines;    Phenolic compounds;    Sorghum;    Tannin compounds;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/546/4/042045/pdf
DOI  :  10.1088/1757-899X/546/4/042045

学科分类：自然科学（综合）

来源: IOP

【 摘 要 】

G5 and G8 are two sorghum mutant lines projected as sorghum varieties for ruminant's feed. However, most of the sorghum panicles contains phenolic compounds, especially in the form of condensed tannins. The objective of this research was to investigate the phenolic and tannin compounds from the panicles of two sorghum mutant lines. The second objective was to identify tannin content by Fourier Transform Mid-Infrared (FTIR) Spectroscopy in the sorghum panicles. The two mutant lines were compared with Pahat variety. This research was arranged into a randomized complete block design with two factors. The first factor was the sorghum variety/mutant lines, whereas the second factor was the generative phase of harvesting time (flowering, soft dough and hard dough). The results showed that the panicles of G5 sorghum contained the lowest total phenol compounds (P-1. At 1521-1514 and 1446-1408 cm-1, a band due to the C-C aromatic compounds was observed. Based on those findings, it can be concluded that G5 and G8 mutant lines produced less total phenol and total tannin contents than Pahat variety. Meanwhile, FTIR could be used to describe the profile of tannin compounds qualitatively.