The thiol-disulfide exchange activity of AtPDI1 is involved in the response to abiotic stresses

BMC Plant Biology

Li Yuan¹  Zhou Zhou¹  Shizhong Zhang¹  Qinghua Sun¹  Xiaoyun Wang¹  Mengyu Wang¹  Ying Lu² 

[1] College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, 271018, Taian, Shandong, People’s Republic of China;College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, 271018, Taian, Shandong, People’s Republic of China;Institute of Shandong River Wetlands, 271100, Jinan, Shandong, People’s Republic of China;

关键词: At;    Arabidopsis;    Abiotic stress;    ABA signaling;    ROS elimination;   

DOI  :  10.1186/s12870-021-03325-7

来源: Springer

【 摘 要 】

BackgroundArabidopsis protein disulfide isomerase 1 (AtPDI1) has been demonstrated to have disulfide isomerase activity and to be involved in the stress response. However, whether the anti-stress function is directly related to the activities of thiol-disulfide exchange remains to be elucidated.ResultsIn the present study, encoding sequences of AtPDI1 of wild-type (WT) and double-cysteine-mutants were transformed into an AtPDI1 knockdown Arabidopsis line (pdi), and homozygous transgenic plants named pdi-AtPDI1, pdi-AtPDI1m1 and pdi-AtPDI1m2 were obtained. Compared with the WT and pdi-AtPDI1, the respective germination ratios of pdi-AtPDI1m1 and pdi-AtPDI1m2 were significantly lower under abiotic stresses and exogenous ABA treatment, whereas the highest germination rate was obtained with AtPDI1 overexpression in the WT (WT- AtPDI1). The root length among different lines was consistent with the germination rate; a higher germination rate was observed with a longer root length. When seedlings were treated with salt, drought, cold and high temperature stresses, pdi-AtPDI1m1, pdi-AtPDI1m2 and pdi displayed lower survival rates than WT and AtPDI1 overexpression plants. The transcriptional levels of ABA-responsive genes and genes encoding ROS-quenching enzymes were lower in pdi-AtPDI1m1 and pdi-AtPDI1m2 than in pdi-AtPDI1.ConclusionTaken together, these results clearly suggest that the anti-stress function of AtPDI1 is directly related to the activity of disulfide isomerase.

【 授权许可】

CC BY