Yi Qi Qing Re Gao formula ameliorates puromycin aminonucleoside-induced nephrosis by suppressing inflammation and apoptosis

BMC Complementary and Alternative Medicine

Research Article

Yumin Wen¹  Yongli Zhan²  Liping Yang²  Huijie Liu³  Xi Dong⁴  Ping Li⁴  Tingting Zhao⁴  Haojun Zhang⁴ 

[1] Beijing University of Chinese Medicine, 10029, Beijing, China;Institute of Clinical Medical Science, China–Japan Friendship Hospital, 10029, Beijing, China;Beijing Key Lab for Immune-Mediated Inflammatory Diseases, 10029, Beijing, China;Department of Nephrology, Guang’anmen Hospital of China Academy of Traditional Chinese Medical Sciences, 100053, Beijing, China;Department of Nephrology, Shunyi Hospital of Traditional Chinese Medicine, 101300, Beijing, China;Institute of Clinical Medical Science, China–Japan Friendship Hospital, 10029, Beijing, China;Beijing Key Lab for Immune-Mediated Inflammatory Diseases, 10029, Beijing, China;

关键词: Apoptosis;    Inflammation;    Puromycin aminonucleoside nephrosis;    Tumor necrosis factor;    YQQRG formula;   

DOI  :  10.1186/s12906-015-0673-9

 received in 2014-11-22, accepted in 2015-05-13,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundYi Qi Qing Re Gao (YQQRG) formula is a traditional Chinese herbal medicine used to treat chronic nephritis. This study was designed to evaluate the underlying mechanism in the use of YQQRG formula to treat nephrosis induced by puromycin aminonucleoside (PAN).MethodsThirty-six male Wistar rats were randomly divided into 3 groups of 12 rats each: a sham group, a vehicle-treated PAN model group (PAN), and a group treated with YQQRG (PAN + YQQRG). The PAN model was established by a single intravenous injection of PAN at a dose of 40 mg/kg body weight; rats in the sham group received the same volume of saline. Twenty-four hour urinary protein was measured 0, 3, 5, 10, and 15 days after the injection. The rats were sacrificed on day 10 and day 15 and the serum lipid profile examined. The renal cortex of each rat was stained with periodic acid–Schiff reagent and the pathologic alterations and ultrastructural changes were examined by transmission electron microscopy. In situ cell apoptosis was detected by a terminal deoxynucleotidyl transferase-mediated uridine 5′-triphosphate-biotin nick end-labeling (TUNEL) assay. Transcriptive levels of inflammatory markers and molecules associated with apoptosis were detected by a real-time polymerase chain reaction and expression of proteins was examined by either immunohistochemistry or Western blot analysis.ResultsYQQRG significantly decreased urinary protein level, and lowered serum lipid level. YQQRG also attenuated histologic lesions in the rat kidneys. Activation of inflammatory markers was largely restored by the administration of YQQRG. TUNEL assay showed that YQQRG decreased the number of apoptotic cells. Both mRNA and protein levels of caspase-3 were significantly reduced in the group treated with YQQRG, whereas expression of the Bcl-2 protein increased in the YQQRG group.ConclusionsYQQRG alleviated kidney injury in PAN-treated rats, possibly through anti-inflammatory and anti-apoptotic effects.

【 授权许可】

Unknown   
© Wen et al.; licensee BioMed Central. 2015. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.