Alpinia oxyphylla Miq. fruit extract activates IGFR-PI3K/Akt signaling to induce Schwann cell proliferation and sciatic nerve regeneration

BMC Complementary and Alternative Medicine

Research Article

Ping-Ling Chiu¹  Ray-Jade Chen²  Hung-Jen Lin³  Chi-Xin Ye⁴  Chien-Chung Lin⁵  Hen-Hong Chang⁶  Yueh-Sheng Chen⁷  Tsung-Jung Ho⁸  Chih-Yang Huang⁹  Chin-Chuan Tsai^1,10  Yung-Ming Chang^1,11 

[1]1PT Biotechnology Co., Ltd., 433, Taichung, Taiwan

[2]Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan

[3]Division of Chinese Internal Medicine, China Medical University Hospital, 404, Taichung, Taiwan

[4]School of Post-Baccalaureate Chinese Medicine, China Medical University, 404, Taichung, Taiwan

[5]Graduate Institute of Basic Medical Science, China Medical University, No 91, Hsueh-Shih Road, 404, Taichung, Taiwan

[6]Orthopaedic Department Armed Forces General Hospital, Taichung, Taiwan

[7]Research Center for Chinese Medicine & Acupuncture, China Medical University, 404, Taichung, Taiwan

[8]Departments of Chinese Medicine, China Medical University Hospital, 404, Taichung, Taiwan

[9]School of Post-Baccalaureate Chinese Medicine, College of Chinese Medicine, China Medical University, 404, Taichung, Taiwan

[10]School of Chinese Medicine, China Medical University, Taichung, Taiwan

[11]School of Chinese Medicine, China Medical University, Taichung, Taiwan

[12]Chinese Medicine Department, China Medical University Beigang Hospital, 651, Beigang, Yunlin, Taiwan

[13]School of Chinese Medicine, China Medical University, Taichung, Taiwan

[14]Graduate Institute of Basic Medical Science, China Medical University, No 91, Hsueh-Shih Road, 404, Taichung, Taiwan

[15]Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan

[16]The School of Chinese Medicine for Post-Baccalaureate, I-Shou University, 840, Kaohsiung, Taiwan

[17]Chinese Medicine Department E-DA Hospital, 824, Kaohsiung, Taiwan

[18]The School of Chinese Medicine for Post-Baccalaureate, I-Shou University, 840, Kaohsiung, Taiwan

[19]Chinese Medicine Department E-DA Hospital, 824, Kaohsiung, Taiwan

[20]1PT Biotechnology Co., Ltd., 433, Taichung, Taiwan

关键词: Alpinia oxyphylla;    IGF-1;    nerve regeneration;    proliferation;    RSC96 Schwann cell;   

DOI  :  10.1186/s12906-017-1695-2

 received in 2016-06-23, accepted in 2017-03-17,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundIt is known that the medicinal herb Alpinia oxyphylla Miq. is widely used as a remedy for diarrhea as well as the symptoms accompanying hypertension and cerebrovascular disorders. Moreover, it has also been reported that Alpinia oxyphylla Miq. has beneficial effects on anti-senescence and neuro-protection. This study focuses on the molecular mechanisms by which the Alpinia oxyphylla Miq. fruits promote neuron regeneration.MethodsA piece of silicone rubber was guided across a 15 mm gap in the sciatic nerve of a rat. This nerve gap was then filled with various doses of Alpinia oxyphylla Miq. fruits to assess their regenerative effect on damaged nerves. Further, we investigated the role of Alpinia oxyphylla Miq. fruits in RSC96 Schwann cell proliferation.ResultsOur current results showed that treatment with the extract of Alpinia oxyphylla Miq. fruits triggers the phosphorylated insulin-like growth factor-1 receptor- phosphatidylinositol 3-kinase/serine-threonine kinase pathway, and up-regulated the proliferating cell nuclear antigen in a dose-dependent manner. Cell cycle analysis on RSC96 Schwann cells showed that, after exposure to Alpinia oxyphylla Miq. fruit extract, the transition from the first gap phase to the synthesis phase occurs in 12–18 h. The expression of the cell cycle regulatory proteins cyclin D1, cyclin E and cyclin A increased in a dose-dependent manner. Transfection with a small interfering RNA blocked the expression of phosphatidylinositol 3-kinase and induced down-regulation both on the mRNA and protein levels, which resulted in a reduction of the expression of the survival factor B-cell lymphoma 2.ConclusionWe provide positive results that demonstrate that Alpinia oxyphylla Miq. fruits facilitate the survival and proliferation of RSC96 cells via insulin-like growth factor-1 signaling.

【 授权许可】

CC BY   
© The Author(s). 2017