Puerarin exhibits greater distribution and longer retention time in neurons than astrocytes in a co-cultured system

Neural Regeneration Research

关键词: spinal cord injury;    propriospinal system;    neural plasticity;    fiber sprouting;    neural repair;    compensation;    regeneration;    propriospinal detours;    neurotrophic factors;    cell-adhesive ligands;    dorsal root ganglia;    L1CAM;    nerve growth factor;    biomaterials;    elastin-like proteins;    Alzheimer′s disease;    AMPK;    apoptosis;    autophagy;    central nervous system;    CCN4;    EGF;    diabetes mellitus;    erythropoietin;    EPO;    FGF;    IGF-1;    mTOR;    neuron;    neuropathy;    oxidative stress;    psychiatric;    stem cells;    WISP1;    Wnt;    peripheral nerve injury;    nerve graft;    nerve conduit;    Wallerian degeneration;    neurotrophic factors;    veins;    autografts;    nerve regeneration;    nerve regeneration;    neuroprotection;    resveratrol;    cerebral ischemia;    cerebral infarction;    matrix metalloproteinase;    molecular docking;    extracellular matrix;    neural regeneration;    nerve regeneration;    vagus nerve stimulation;    cerebral ischemia;    inflammatory cytokines;    infarct volume;    neurological function;    NSFC grants;    neural regeneration;    nerve regeneration;    Xingnao Kaiqiao needling method;    ischemic stroke;    randomized controlled trial;    systemic reviews;    meta-analysis;    long-term efficacy;    mortality;    recurrence;    disability;    adverse reactions;    health economics indicators;    neural regeneration;    nerve regeneration;    DDPH;    cerebral ischemia;    hippocampus;    blood flow;    isolated basilar artery;    dose-response curve;    NSFC grant;    neural regeneration;    nerve regeneration;    traumatic brain injury;    coma;    median nerve electrical stimulation;    wake-promoting;    orexin-A;    OX1R;    NSFC grants;    neural regeneration;    nerve regeneration;    seizure;    antiepileptic drugs;    immature brain;    hippocampus;    synaptic plasticity;    glutamate receptor;    NSFC grant;    neural regeneration;    nerve regeneration;    puerarin;    in vitro experiments;    co-culture;    neurons;    astrocytes;    Transwell;    neonatal rats;    neural regeneration;   

DOI  :  10.4103/1673-5374.155435

来源: DOAJ

【 摘 要 】

The phytoestrogen puerarin has been shown to protect neurons and astrocytes in the brain, and is therefore an attractive drug in the treatment of Alzheimer′s disease, Parkinson′s disease and cerebral ischemia. Whether puerarin exhibits the same biological processes in neurons and astrocytes in vitro has rarely been reported. In this study, cortical neurons and astrocytes of newborn Sprague-Dawley rats were separated, identified and co-cultured in a system based on Transwell membranes. The retention time and distribution of puerarin in each cell type was detected by fluorescence spectrophotometry and fluorescence microscope. The concentration of puerarin in both co-cultured and separately cultured neurons was greater than that of astrocytes. Puerarin concentration reached a maximum 20 minutes after it was added. At 60 minutes after its addition, a scant amount of drug was detected in astrocytes; however in both separately cultured and co-cultured neurons, the concentration of puerarin achieved a stable level of about 12.8 ng/mL. The results indicate that puerarin had a higher concentration and longer retention time in neurons than that observed in astrocytes.

【 授权许可】

Unknown