Enhancement of Calibrachoa growth, secondary metabolites and bioactivity using seaweed extracts

BMC Complementary and Alternative Medicine

Research Article

Jeff Norrie¹  Hosam O. Elansary²  Hayssam M. Ali³  Kowiyou Yessoufou⁴  Eman A. Mahmoud⁵  Mohamed Z. M. Salem⁶ 

[1] Acadian Seaplants Limited, 30 Brown Avenue, Dartmouth, Nova Scotia, Canada;Biodiversity Institute of Ontario, University of Guelph, N1G 2W1, Guelph, Ontario, Canada;Department of Floriculture, Ornamental Horticulture and Garden Design, Faculty of Agriculture (El-Shatby), Alexandria University, Alexandria, Egypt;Botany and Microbiology Department, College of Science, King Saud University, P.O. Box 2455, 11451, Riyadh, Saudi Arabia;Timber Trees Research Department, Sabahia Horticulture Research Station, Agriculture Research Center, Horticulture Research Institute, Alexandria, Egypt;Department of Environmental Sciences, University of South Africa, Florida campus, 1710, Florida, South Africa;Department of Food Technology, Damietta University, Damietta, Egypt;Forestry and Wood Technology Department, Faculty of Agriculture (EL-Shatby), Alexandria University, Alexandria, Egypt;

关键词: Calibrachoa;    Seaweed extract;    Antifungal;    Antibacterial;    Antioxidants;   

DOI  :  10.1186/s12906-016-1332-5

 received in 2016-02-15, accepted in 2016-08-31,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundCalibrachoa x hybrida (Solanaceae) cultivars are widely used in North and South America as ornamental plants. Their potential as a source of antimicrobial compounds might be enhanced by seaweed extract (SWE) applications.MethodsSWE of Ascophyllum nodosum were applied at 5 and 7 ml/L as a soil drench or foliar spray on Calibrachoa cultivars of Superbells® ‘Dreamsicle’ (CHSD) and Superbells® ‘Frost Fireʼ (CHSF). The total phenolics, tannins and antioxidants composition as well as specific flavonols in leaf extracts were determined. Further, the chemical composition of SWE was assessed.ResultsThe drench and foliar SWE treatments significantly enhanced Calibrachoa cultivars leaf number and area, dry weight, plant height, antioxidant capacity as well as phenolic, flavonols and tannin content. The increased growth and composition of phenols, flavonols and tannins was attributed to the stimulatory effects of SWE mineral composition. The antifungal activity of Calibrachoa cultivars was significantly enhanced following SWE treatments and the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were in the range of 0.07–0.31 mg/ml and from 0.16 to 0.56 mg/ml, respectively. Moreover, antibacterial activity was significantly increased and the MIC and minimum bactericidal concentration (MBC) measurements were in the range of 0.06–0.23 mg/ml and from 0.10 to 0.44 mg/ml, respectively. The most sensitive fungus to SWE treatments was C. albicans and the most sensitive bacterium was E. cloacae.ConclusionThe results suggest that enhanced antifungal and antibacterial activities might be attributed to significant increases of phenolic, flavonols and tannin contents, which ultimately enhance the potential of Calibrachoa as a natural source of alternative antibiotics.

【 授权许可】

CC BY   
© The Author(s). 2016