Antimutagenic and anticancer activity of Darjeeling tea in multiple test systems

BMC Complementary and Alternative Medicine

Research Article

Shanta Adak¹  Ashok K Giri²  Udayan Bhattacharya²  Niladri Shekhar Majumder²  Biswajit Bera³ 

[1] Department of Zoology, Seth Anandram Jaipuria College, Kolkata, India;Molecular and Human Genetics Division, Indian Institute of Chemical Biology, 4, Raja S. C. Mullick Road, 700 032, JadavpurKolkata, India;Tea Board, Kolkata, India;

关键词: Darjeeling tea;    Antimutagenic;    Anticancer;    Apoptosis;   

DOI  :  10.1186/1472-6882-14-327

 received in 2014-03-12, accepted in 2014-08-11,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundDarjeeling tea, a most popular variety of black tea, though consumed by the people in different parts of world but its beneficial health effects have not been investigated in details. In this study, the antimutagenic and anticancer effect of Darjeeling tea extract (DTE) has been evaluated.MethodsAntimutagenic activity of the DTE was carried out in two different strains of Salmonella typhimurium by AMES test against a known mutagen benzo[a]pyrene (B[a]P) with S9 activation. Moreover, anticlastogenic property of DTE was also measured by micronuclei formation (MN) against B[a]P with S9 activation in human lymphocytes. The anticancer activity of the same was studied on U937 cell line. Here, Human PBMCs were used as the normal cell control to identify selective anticancer activity of the extract against U937 cells.ResultsThe results showed significant antimutagenic activity on bacterial strains. A significant decrease in MN was also observed in the DTE treated human lymphocyte cultures pretreated with B[a]P when compared with B[a]P treated cultures alone. The study clearly exhibited anticancer activity of the extract on U937 cell line. Further studies also revealed that apoptosis induction is an important mechanism behind the anticancer effect of DTE.ConclusionOverall, this study indicates that DTE has significant antimutagenic and anticancer activities on bacterial and mammalian cells respectively.

【 授权许可】

Unknown   
© Bhattacharya et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.