Antimutagenic and anticancer activity of Darjeeling tea in multiple test systems

BMC Complementary and Alternative Medicine

Ashok K Giri¹  Biswajit Bera³  Niladri Shekhar Majumder¹  Shanta Adak²  Udayan Bhattacharya¹ 

[1] Molecular and Human Genetics Division, Indian Institute of Chemical Biology, 4, Raja S. C. Mullick Road, Jadavpur, 700 032 Kolkata, India;Department of Zoology, Seth Anandram Jaipuria College, Kolkata, India;Tea Board, Kolkata, India

关键词: Apoptosis;    Anticancer;    Antimutagenic;    Darjeeling tea;   

Others  :  1086812
DOI  :  10.1186/1472-6882-14-327

 received in 2014-03-12, accepted in 2014-08-11,  发布年份 2014

【 摘 要 】

Background

Darjeeling tea, a most popular variety of black tea, though consumed by the people in different parts of world but its beneficial health effects have not been investigated in details. In this study, the antimutagenic and anticancer effect of Darjeeling tea extract (DTE) has been evaluated.

Methods

Antimutagenic activity of the DTE was carried out in two different strains of Salmonella typhimurium by AMES test against a known mutagen benzo[a]pyrene (B[a]P) with S9 activation. Moreover, anticlastogenic property of DTE was also measured by micronuclei formation (MN) against B[a]P with S9 activation in human lymphocytes. The anticancer activity of the same was studied on U937 cell line. Here, Human PBMCs were used as the normal cell control to identify selective anticancer activity of the extract against U937 cells.

Results

The results showed significant antimutagenic activity on bacterial strains. A significant decrease in MN was also observed in the DTE treated human lymphocyte cultures pretreated with B[a]P when compared with B[a]P treated cultures alone. The study clearly exhibited anticancer activity of the extract on U937 cell line. Further studies also revealed that apoptosis induction is an important mechanism behind the anticancer effect of DTE.

Conclusion

Overall, this study indicates that DTE has significant antimutagenic and anticancer activities on bacterial and mammalian cells respectively.

【 授权许可】

   
2014 Bhattacharya et al.; licensee BioMed Central Ltd.

【 图 表 】

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