Antispasmodic, bronchodilator, vasorelaxant and cardiosuppressant effects of Buxus papillosa

BMC Complementary and Alternative Medicine

Research Article

Anwarul-Hassan Gilani¹  Manzoor Ahmed²  Arif-ullah Khan³  Muhammad Iqbal Choudhary⁴  Shamsher Ali⁵ 

[1] Department of Biological and Biomedical Sciences, The Aga Khan University Medical College, 74800, Karachi, Pakistan;Pakistan Council of Science and Technology, Islamabad, Pakistan;Department of Chemistry, University of Malakand, Chakdara, Dir (L), Pakistan;Department of Pharmacology, Riphah Institute of Pharmaceutical Sciences, Riphah International University, Islamabad, Pakistan;International Center for Chemical and Biological Sciences, H EJ Research Institute of Chemistry, University of Karachi, Karachi, Pakistan;Molecular and Integrative Physiological Sciences, Harvard School of Public Health, Boston, MA, USA;

关键词: Buxus papillosa;    Antispasmodic;    Bronchodilator;    Vasodilator;    Cardio-depressant;    Ca antagonist;   

DOI  :  10.1186/s12906-017-1558-x

 received in 2016-05-05, accepted in 2017-01-05,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThe present research was carried out to investigate pharmacological properties of Buxus papillosa C.K. Schneid. (Buxaceae).MethodsBuxus papillosa extracts of leaves (BpL), stem (BpS), roots (BpR) and BpL fractions: hexane (BpL-H), aqueous (BpL-A) also plant constituent, cyclomicrobuxine effect were studied in jejunum, atria, aorta and tracheal preparations from rabbit and guine-peg.ResultsCa++ antagonistic effect of BpS, BpR, BpL-H, BpL-A and cyclomicrobuxine were conclusively suggested, when spontaneous contractions of rabbit jejunal preparation was relaxed along with subsequent relaxation of potassium chloride (80 mM) induced contractions. Ca++ antagonistic effect was further confirmed, when a prominent right shift like that of verapamil was observed in Ca++ concentration-response curves, drawn in a tissue pretreated with BpL (0.3–1.0 mg/mL). In rabbit tracheal tissues BpL, BpS, BpR, BpL-H and BpL-A produced a prominent relaxation in contractions induced by potassium chloride (80 mM) and carbachol (1 μm). When tested in rabbit aortic rings, BpL, BpS, BpR, BpL-H and BpL-A showed concentration-dependent (0.1–3.0 mg/mL) vasorelaxant effect against phenylephrine (1 μM) and high K+-induced contractions. In isolated guinea-pig right atria, BpL, BpS, BpR, BpL-H and BpL-A suppressed atrial force of spontaneous contractions, with BpL-A being most potent.ConclusionsOur results reveal that Buxus papillosa possesses gut, airways and cardiovascular inhibitory actions.

【 授权许可】

CC BY   
© The Author(s). 2017