Mechanistic basis for mitigating drought tolerance by selenium application in tobacco (Nicotiana tabacum L.): a multi-omics approach

Frontiers in Plant Science

Plant Science

Zhong Wang¹  Lidong Teng²  Jinpeng Yang³  Chunlei Yang³  Ruiwei Yang³  Baoming Qiao³  Huaxin Dai⁴  Taibo Liang⁴  Yanling Zhang⁴  Waleed Amjad Khan⁵ 

[1] China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of China National Tobacco Corporation (CNTC), Zhengzhou, China;Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China;Department of Research Center on Tobacco Cultivating and Curing, Tobacco Research Institute of Hubei, Wuhan, China;Department of Tobacco Agriculture, Zhengzhou Tobacco Research Institute of China National Tobacco Corporation (CNTC), Zhengzhou, China;Tasmanian Institute of Agriculture, University of Tasmania, Hobart, TAS, Australia;

关键词: cigar tobacco;    drought;    selenium;    multi-omics;    target genes;   

DOI  :  10.3389/fpls.2023.1255682

 received in 2023-07-09, accepted in 2023-09-04,  发布年份 2023

来源: Frontiers

【 摘 要 】

The lack of irrigation water in agricultural soils poses a significant constraint on global crop production. In-depth investigation into microRNAs (miRNAs) has been widely used to achieve a comprehensive understanding of plant defense mechanisms. However, there is limited knowledge on the association of miRNAs with drought tolerance in cigar tobacco. In this study, a hydroponic experiment was carried out to identify changes in plant physiological characteristics, miRNA expression and metabolite profile under drought stress, and examine the mitigating effects of selenium (Se) application. The shoot dry weight of drought-stressed plants was approximately half (50.3%) of that in non-stressed (control) conditions. However, plants supplied with Se attained 38.8% greater shoot dry weight as compared to plants with no Se supply under drought stress. Thirteen miRNAs were identified to be associated with drought tolerance. These included 7 known (such as nta-miR156b and nta-miR166a) and 6 novel miRNAs (such as novel-nta-miR156-5p and novel-nta-miR209-5p) with the target genes of squamosa promoter-binding-like protein 4 (SPL4), serine/threonine protein phosphatase 2A (PPP2A), cation/calcium exchanger 4-like (CCX4), extensin-1-like (EXT1) and reduced wall acetylation 2 (RWA2). Further investigation revealed that the expression levels of Ext1 and RWA2 were significantly decreased under drought stress but increased with Se addition. Moreover, key metabolites such as catechin and N-acetylneuraminic acid were identified, which may play a role in the regulation of drought tolerance. The integrated analysis of miRNA sequencing and metabolome highlighted the significance of the novel-nta-miR97-5p- LRR-RLK- catechin pathway in regulating drought tolerance. Our findings provide valuable insights into the molecular mechanisms underlying drought tolerance and Se-induced stress alleviation in cigar tobacco.

【 授权许可】

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Copyright © 2023 Dai, Yang, Teng, Wang, Liang, Khan, Yang, Qiao, Zhang and Yang