Effects of ecological environment and host genotype on the phyllosphere bacterial communities of cigar tobacco (Nicotiana tabacum L.)

Ecology and Evolution

Lei Xing¹  Juan Li¹  Zhenxie Yi¹  Yuhong Jia²  Yi Liu²  Heng Xu²  Xi Hu²  Huaqun Yin³  Jianlei Yang³ 

[1] College of Agronomy Hunan Agricultural University Changsha China;Greatwall Cigar Factory China Tobacco Sichuan Industrial Co., Ltd Shifang China;School of Minerals Processing and Bioengineering Central South University Changsha China;

关键词: bacterial communities;    cigar tobacco;    environmental conditions;    genotype;    phyllosphere;    species diversity;   

DOI  :  10.1002/ece3.7861

来源: DOAJ

【 摘 要 】

Abstract Microorganisms of plant phyllosphere play an important role in plant health and productivity and are influenced by abiotic and biotic factors. In this study, we investigated the phyllosphere bacterial communities of three cigar tobacco varieties cultivated in Guangcun (GC) and Wuzhishan (WZS), Hainan, China. Metagenomic DNA was extracted from tobacco leaf samples and sequenced by 16S rDNA amplicon sequencing. Our results showed that bacterial communities of cigar tobacco phyllosphere in GC exhibited remarkably higher alpha diversity than that in WZS. There was slight effect of tobacco genotype variations on the alpha diversity in both cultivation sites, and beta diversity and structure of bacterial community were not influenced significantly by the cultivation sites and tobacco varieties. Statistical analyses of species diversity unraveled that the dominant species in bacterial communities of cigar tobacco phyllosphere among all these samples were phylogenetically affiliated to Proteobacteria and Cyanobacteria. At the genus level, the most abundant microorganism was Limnobacter, followed by Brevundimonas, unidentified_Cyanobacteria, and Pseudomonas. Additionally, environmental conditions except for humidity were negatively correlated with the relative abundance of bacterial genera. Further analyses revealed that influence of site‐specific factors on tobacco bacterial community was relatively higher than genotype‐specific factors. In short, this study may contribute to the knowledge base of practical applications of bacterial inoculants for tobacco leaf production.

【 授权许可】

Unknown