Composition and diversity of gut microbiota across developmental stages of Spodoptera frugiperda and its effect on the reproduction

Frontiers in Microbiology

Microbiology

Ximei Huang¹  Boyang Guan¹  Qili Feng¹  Junrui Fu¹  Junhan Wang¹  Huimin Deng² 

[1] Guangdong Key Laboratory of Insect Developmental Biology and Applied Technology, Guangzhou Key Laboratory of Insect Development Regulation and Application Research, Institute of Insect Science and Technology and School of Life Sciences, South China Normal University, Guangzhou, China;Guangdong Key Laboratory of Insect Developmental Biology and Applied Technology, Guangzhou Key Laboratory of Insect Development Regulation and Application Research, Institute of Insect Science and Technology and School of Life Sciences, South China Normal University, Guangzhou, China;Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China;

关键词: Spodoptera frugiperda;    gut microbiota;    microbiome;    16S rDNA;    reproduction;   

DOI  :  10.3389/fmicb.2023.1237684

 received in 2023-06-09, accepted in 2023-08-31,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionSpodoptera frugiperda is a serious world-wide agricultural pest. Gut microorganisms play crucial roles in growth, development, immunity and behavior of host insects.MethodsHere, we reported the composition of gut microbiota in a laboratory-reared strain of S. frugiperda using 16S rDNA sequencing and the effects of gut microbiota on the reproduction.ResultsProteobacteria and Firmicutes were the predominant bacteria and the taxonomic composition varied during the life cycle. Alpha diversity indices indicated that the eggs had higher bacterial diversity than larvae, pupae and adults. Furthermore, eggs harbored a higher abundance of Ralstonia, Sediminibacterium and microbes of unclassified taxonomy. The dynamics changes in bacterial communities resulted in differences in the metabolic functions of the gut microbiota during development. Interestingly, the laid eggs in antibiotic treatment groups did not hatch much due to the gut dysbacteriosis, the results showed gut microbiota had a significant impact on the male reproduction.DiscussionOur findings provide new perspectives to understand the intricate associations between microbiota and host, and have value for the development of S. frugiperda management strategies focusing on the pest gut microbiota.

【 授权许可】

Unknown   
Copyright © 2023 Fu, Wang, Huang, Guan, Feng and Deng.