Anti-osteoporotic drugs affect the pathogenesis of gut microbiota and its metabolites: a clinical study

Frontiers in Cellular and Infection Microbiology

Cellular and Infection Microbiology

Zhi-neng Chen¹  Lin-pu Ge¹  Gui-jin Li¹  Yang Zhang¹  Feng Cheng¹  Kun Yan¹  Xiao-gang Chen¹  Xin-miao Yao¹  Hai-feng Chen²  Rui-kun Zhang² 

[1] Department of Orthopedics, The Third Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China;The Third Clinical Medical College of Zhejiang Chinese Medical University, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China;

关键词: gut microbiota;    16S rDNA;    untargeted metabolism;    osteoporosis;    clinical study;   

DOI  :  10.3389/fcimb.2023.1091083

 received in 2022-11-06, accepted in 2023-05-22,  发布年份 2023

来源: Frontiers

【 摘 要 】

BackgroundDisordered gut microbiota (GM) structure and function may contribute to osteoporosis (OP). This study explores how traditional Chinese medicine (TCM) intervention affects the structure and function of the GM in patients with OP.MethodIn a 3-month clinical study, 43 patients were randomly divided into two groups receiving conventional treatment and combined TCM (Yigu decoction, YGD) treatment. The correlation between the intestinal flora and its metabolites was analyzed using 16S rDNA and untargeted metabolomics and the combination of the two.ResultsAfter three months of treatment, patients in the treatment group had better bone mineral density (BMD) than those in the control group (P < 0.05). Patients in the treatment group had obvious abundance changes in GM microbes, such as Bacteroides, Escherichia-Shigella, Faecalibacterium, Megamonas, Blautia, Klebsiella, Romboutsia, Akkermansia, and Prevotella_9. The functional changes observed in the GM mainly involved changes in metabolic function, genetic information processing and cellular processes. The metabolites for which major changes were observed were capsazepine, Phe-Tyr, dichlorprop, D-pyroglutamic acid and tamsulosin. These metabolites may act through metabolic pathways, the citrate cycle (TCA cycle) and beta alanine metabolism. Combined analysis showed that the main acting metabolites were dichlorprop, capsazepine, D-pyroglutamic acid and tamsulosin.ConclusionThis study showed that TCM influenced the structure and function of the GM in patients with OP, which may be one mechanism by which TCM promotes the rehabilitation of patients with OP through the GM.

【 授权许可】

Unknown   
Copyright © 2023 Zhang, Yan, Chen, Zhang, Li, Chen, Ge, Cheng, Chen and Yao